Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea

Type Article
Date 2017-11
Language English
Author(s) Da Costa Fiz1, 5, Le Grand Fabienne2, Quere Claudie1, Bougaran GaelORCID3, Cadoret Jean-Paul3, Robert ReneORCID1, 4, Soudant Philippe2
Affiliation(s) 1 : IFREMER, Lab Sci Environm Marin, UMR 6539, LEMAR, F-29280 Plouzane, France.
2 : UBO, IUEM, Lab Sci Environm Marin, UMR 6539,LEMAR, Technopole Brest Iroise, Plouzane, France.
3 : IFREMER, Lab Physiol & Biotechnol Algues, Rue Ile Yeu,BP 21105, F-44311 Nantes 3, France.
4 : IFREMER, Unite Littoral, Ctr Bretagne, ZI Pointe Diable CS 10070, F-29280 Plouzane, France.
5 : Oceano Fresco Lda, Edifcio Mira Ctr,Rua Matadouro,Piso 2,Lab B2, P-3070436 Valeirinha, Mira, Portugal.
Source Algal Research-biomass Biofuels And Bioproducts (2211-9264) (Elsevier Science Bv), 2017-11 , Vol. 27 , P. 177-189
DOI 10.1016/j.algal.2017.09.003
WOS© Times Cited 34
Keyword(s) Tisochrysis lutea, Nitrogen limitation, Growth phase, Flow cytometry, Fatty acids, Lipid classes

Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates were enhanced at stationary phase in both strains, regardless of N supply. The oleaginous strain (T +) accumulated triacylglycerols; whereas, the standard strain T accumulated alkenones as reserve lipids, especially in N-reduced medium and stationary phase. Each T. lutea strain exhibited an intrinsic specific FA profile in neutral lipids (NL) and, to a lesser extent, in polar lipids (PL) independent of N supply and growth phase. T + contained more saturated and monounsaturated fatty acids (especially 14:0 and 18:1n-9), but less polyunsaturated fatty acids (18:4n-3 and 22:6n-3) than T. Overall, growth phase induced more changes in fatty acid profiles of both T. lutea strains in NL and PL than N supply in culture medium.

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Da Costa Fiz, Le Grand Fabienne, Quere Claudie, Bougaran Gael, Cadoret Jean-Paul, Robert Rene, Soudant Philippe (2017). Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea. Algal Research-biomass Biofuels And Bioproducts, 27, 177-189. Publisher's official version : https://doi.org/10.1016/j.algal.2017.09.003 , Open Access version : https://archimer.ifremer.fr/doc/00399/51074/