Continuous selection pressure to improve temperature acclimation of Tisochrysis lutea

Type Article
Date 2017-09
Language English
Author(s) Bonnefond Hubert1, Grimaud Ghjuvan2, Rumin Judith3, Bougaran GaelORCID3, Talec Amelie1, Gachelin Manon1, Boutoute Marc1, Pruvost Eric1, Bernard Olivier2, Sciandra Antoine1
Affiliation(s) 1 : UPMC Univ Paris 06, Sorbonne Univ, CNRS, INSU,LOV, Villefranche Sur Mer, France.
2 : INRIA, BIOCORE, Sophia Antipolis, France.
3 : PBA, IFREMER, Nantes, France.
Source Plos One (1932-6203) (Public Library Science), 2017-09 , Vol. 12 , N. 9 , P. e0183547 (1-19)
DOI 10.1371/journal.pone.0183547
WOS© Times Cited 21

Temperature plays a key role in outdoor industrial cultivation of microalgae. Improving the thermal tolerance of microalgae to both daily and seasonal temperature fluctuations can thus contribute to increase their annual productivity. A long term selection experiment was carried out to increase the thermal niche (temperature range for which the growth is possible) of a neutral lipid overproducing strain of Tisochrysis lutea. The experimental protocol consisted to submit cells to daily variations of temperature for 7 months. The stress intensity, defined as the amplitude of daily temperature variations, was progressively increased along successive selection cycles. Only the amplitude of the temperature variations were increased, the daily average temperature was kept constant along the experiment. This protocol resulted in a thermal niche increase by 3 degrees C (+16.5%), with an enhancement by 9% of the maximal growth rate. The selection process also affected T. lutea physiology, with a feature generally observed for 'cold-temperature' type of adaptation. The amount of total and neutral lipids was significantly increased, and eventually productivity was increased by 34%. This seven month selection experiment, carried out in a highly dynamic environment, challenges some of the hypotheses classically advanced to explain the temperature response of microalgae.

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Bonnefond Hubert, Grimaud Ghjuvan, Rumin Judith, Bougaran Gael, Talec Amelie, Gachelin Manon, Boutoute Marc, Pruvost Eric, Bernard Olivier, Sciandra Antoine (2017). Continuous selection pressure to improve temperature acclimation of Tisochrysis lutea. Plos One, 12(9), e0183547 (1-19). Publisher's official version : , Open Access version :