|Author(s)||Francois Cyrille1, Quellec Stéphane2, Collewet Guylaine2, Peyramaure Paul1, 4, Musse Maja2, Lamy Jean-Baptiste1, Sauriau Pierre-Guy3, Haure Joel1|
|Affiliation(s)||1 : Ifremer, RBE-SG2M-LGPMM, Station de La Tremblade, Avenue de Mus de loup, F-17390 La Tremblade, France
2 : Irstea, IRM Food, 17 avenue de Cucillé, F-35044 Rennes Cedex, France
3 : Université La Rochelle, UMR CNRS/7266, LIENSs, Bâtiment ILE, 2 rue Olympe de Gouges, F-17000 La Rochelle, France
4 : INSA, 20 Avenue des Buttes de Coësmes, F-35708 Rennes Cedex 7, France
|Meeting||Physiomar 17. 18-21 September 2017, Cambridge, UK|
High-throughput phenotyping of shellfish’s traits constitutes a challenge due to the specificities of aquatic animals. Appropriate measuring tools have often to be developed or adapted, in order to continuously and automatically acquire data of biomarkers of interest.
IMAGIGAS project aimed to explore a non-destructive and non-invasive approach by using magnetic resonance imaging (MRI) to monitor at high-throughput two biomarkers (body weight and temperature) of the pacific oyster, before and during infectious challenges by Ostreid Herpesvirus type 1 (OsHV-1).
Pacific oysters and OsHV-1 suspension and contaminated seawater were produced at Ifremer facilities. The experiments were conducted in 2016-2017 by three staffs (Ifremer LGPMM, Irstea IRM Food, Université de La Rochelle LIENSs) at PRISM platform of Irstea at Rennes who provided a Siemens Avanto 1,5 T MRI.
One objective was to monitor body weight of numerous individuals. Protocols were inspired by previous Ifremer / Irstea collaborative works and allowed to increase the throughput of measure to 69 oysters every 45 minutes. The estimation of body weight is based on a relation established between the sum of pixels whose grey level is greater than a particular value in pictures taken by MRI and the weight of drained then lyophilized flesh of oysters measured by precision balance.
Each development experiments were completed by application experiments with infection of oysters by OsHV-1 and measures of body weight.
Francois Cyrille, Quellec Stéphane, Collewet Guylaine, Peyramaure Paul, Musse Maja, Lamy Jean-Baptiste, Sauriau Pierre-Guy, Haure Joel (2017). High-throughput phenotyping of health biomarkers in Crassostrea gigas by magnetic resonance imaging. Physiomar 17. 18-21 September 2017, Cambridge, UK. https://archimer.ifremer.fr/doc/00404/51553/