Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae

Type Article
Date 2017-10
Language English
Author(s) Morga Benjamin1, Renault Tristan2, Faury Nicole1, Lerond Sophie1, Garcia Celine1, Chollet Bruno1, Joly Jean-Pierre1, Lapegue Sylvie1, Harrang Estelle1, Arzul IsabelleORCID1
Affiliation(s) 1 : Inst Francais Rech Exploitat Mer, Lab Genet & Pathol Mollusques Marins, IFREMER, La Tremblade, France.
2 : Inst Francais Rech Exploitat Mer, Dept Ressources Biol & Environm, IFREMER, Nantes, France.
Source Frontiers In Cellular And Infection Microbiology (2235-2988) (Frontiers Media Sa), 2017-10 , Vol. 7 , N. 433 , P. 1-13
DOI 10.3389/fcimb.2017.00433
WOS© Times Cited 7
Keyword(s) Bonamia ostreae, protozoan, Ostrea edulis, resistance, experimental infection, haemocytes, gene expression, apoptosis
Abstract

Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and phagocytosis activity were analyzed by flow cytometry. Four genes homologous to those shown to be involved in immunity were selected (Inhibitor of apotosis OeIAP, Fas ligand OeFas-ligand, Oe-SOD, and OeEc-SOD) and monitored by quantitative reverse-transcription PCR (qRT-PCR). Infected oysters showed higher phagocytosis activity than controls. Infected selected oyster show a lower phagocytosis activity which might be a protection against the parasite infection. The expression of OeIAP and OeFas-ligand gene was significantly increased in selected oysters at 5 days post-injection. OeIAP gene expression appeared to be significantly increased in wild-type oysters at 8 days post-injection. Our results suggest that resistance to bonamiosis partly relies on the ability of the oysters to modulate apoptosis.

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Morga Benjamin, Renault Tristan, Faury Nicole, Lerond Sophie, Garcia Celine, Chollet Bruno, Joly Jean-Pierre, Lapegue Sylvie, Harrang Estelle, Arzul Isabelle (2017). Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae. Frontiers In Cellular And Infection Microbiology, 7(433), 1-13. Publisher's official version : https://doi.org/10.3389/fcimb.2017.00433 , Open Access version : https://archimer.ifremer.fr/doc/00405/51665/