The ZooCAM, a new in-flow imaging system for fast onboard counting, sizing and classification of fish eggs and metazooplankton

Type Article
Date 2018-09
Language English
Author(s) Colas FlorentORCID1, Tardivel Morgan1, Perchoc Jonathan2, Lunven Michel2, Forest Bertrand1, Guyader Gerard3, Danielou Marie-Madeleine2, Le Mestre Sophie4, Bourriau Paul5, Antajan ElvireORCID6, Sourisseau Marc2, Huret MartinORCID4, Petitgas Pierre5, Romagnan Jean-Baptiste5
Affiliation(s) 1 : Ifremer LDCM, Ctr Bretagne, CS 10070, F-29280 Plouzane, France.
2 : Ifremer Dyneco Pelagos, Ctr Bretagne, CS 10070, F-29280 Plouzane, France.
3 : Ifremer SI2M, Ctr Bretagne, CS 10070, F-29280 Plouzane, France.
4 : Ifremer STH LBH, Ctr Bretagne, CS 10070, F-29280 Plouzane, France.
5 : Ifremer EMH, Ctr Atlantique, BP 21105, F-44311 Nantes 03, France.
6 : Ifremer LERBL, Ctr Manche Mer Nord, F-62200 Boulogne Sur Mer, France.
Source Progress In Oceanography (0079-6611) (Pergamon-elsevier Science Ltd), 2018-09 , Vol. 166 , P. 54-65
DOI 10.1016/j.pocean.2017.10.014
WOS© Times Cited 4
Keyword(s) ZooCAM, In-flow imaging, Fish eggs, Zooplankton, Ecosystemic survey
Abstract

In this paper we present the ZooCAM, a system designed to digitize and analyse on board large volume samples of preserved and living metazooplankton (i.e. multicellular zooplankton) and fish eggs > 300 µm ESD. The ZooCAM has been specifically designed to overcome the difficulties to analyse zooplankton and fish eggs in the framework of the PELGAS survey, and provide high frequency data. The ZooCAM fish eggs counts were comparable to those done with a dissecting microscope. The ZooCAM enabled the accurate prediction and fast on board validation of staged anchovy and sardine eggs in almost real time after collection. A comparison with the ZooScan, on a more complex zooplanktonic community, provided encouraging results on the agreement between the 2 instruments. ZooCAM and ZooScan enabled the identification of similar communities and produced similar total zooplankton abundances, size distributions, and size spectra slopes, when tested on the same samples. However these results need to be further refined due to the small number of samples used to compare the two instruments. The main ZooCAM drawback resides in a slight but sensible underestimation of abundances and sizes, and therefore individual and community biovolumes. The ZooCAM have been successfully deployed over 4 years, on numerous surveys without suffering any major failure. When used in line with the CUFES it provided high resolution maps of staged fish eggs and zooplanktonic functional groups. Hence the ZooCAM is an appropriate tool for the development of on board, high frequency, high spatial coverage zooplanktonic and ecosystemic studies.

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Colas Florent, Tardivel Morgan, Perchoc Jonathan, Lunven Michel, Forest Bertrand, Guyader Gerard, Danielou Marie-Madeleine, Le Mestre Sophie, Bourriau Paul, Antajan Elvire, Sourisseau Marc, Huret Martin, Petitgas Pierre, Romagnan Jean-Baptiste (2018). The ZooCAM, a new in-flow imaging system for fast onboard counting, sizing and classification of fish eggs and metazooplankton. Progress In Oceanography, 166, 54-65. Publisher's official version : https://doi.org/10.1016/j.pocean.2017.10.014 , Open Access version : https://archimer.ifremer.fr/doc/00407/51833/