Characterization of the spoilage potential of pure and mixed cultures of bacterial species isolated from tropical yellowfin tuna ( Thunnus albacares )

Type Article
Date 2018-02
Language English
Author(s) Silbande A.1, 2, 3, Cornet Josiane1, Cardinal MireilleORCID1, Chevalier FrederiqueORCID1, Rochefort K.2, Smith-Ravin J.3, Adenet S.2, Leroi Francoise1
Affiliation(s) 1 : IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol E, Nantes, France.
2 : IFREMER, PARM, Lamentin, Martinique, France.
3 : Univ Antilles, Dept Sci Inter Fac DSI, AIHP GEODE EA929, Grp BIOSPHERES, Schoelcher, Martinique, France.
Source Journal Of Applied Microbiology (1364-5072) (Wiley), 2018-02 , Vol. 124 , N. 2 , P. 559-571
DOI 10.1111/jam.13663
WOS© Times Cited 6
Keyword(s) bacterial interactions, bacterial species, fish, Pseudomonas, sensory quality, spoilage potential, tropical, tuna
Abstract

Aim

The spoilage potential of 28 bacterial strains isolated from spoiled raw yellowfin tuna was evaluated.

Methods and Results

Bacterial species were inoculated in irradiated tuna matrix. Chemical changes, bacterial growth and sensory quality were monitored during aerobic storage at 8°C. Pseudomonas spp., Enterobacter spp. and Escherichia hermanii had no spoiling effect. Brochothrix thermosphacta and Carnobacterium divergens/maltaromaticum developed moderate unpleasant odors. Hafnia paralvei and Serratia spp. released strong off-odors (pyrrolidine, sulfur/cabbage). No bacterial group (except H. paralvei) combined with Pseudomonas spp. deteriorated the sensory quality of tuna. When C. divergens/maltaromaticum was associated with H. paralvei or B. thermosphacta, the odor is close to the naturally contaminated tuna stored on the same conditions. The pH, Total Volatile Basic Nitrogen (TVBN) and Trimethylamine (TMA) were not correlated with the spoilage.

Conclusions

The bacterial species had a different impact on the sensory quality of the fish. The bacterial interactions leading to an enhancement or an inhibition of the spoilage potential and the bacterial growth.

Significance and Impact of Study

The Specific Spoilage Organism (SSO) appears to be an association of Lactic Acid Bacteria (LAB) with Enterobacteriaceae or B. thermosphacta. Pseudomonas, often dominant at the sensory rejection time, is not a good quality indicator.

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How to cite 

Silbande A., Cornet Josiane, Cardinal Mireille, Chevalier Frederique, Rochefort K., Smith-Ravin J., Adenet S., Leroi Francoise (2018). Characterization of the spoilage potential of pure and mixed cultures of bacterial species isolated from tropical yellowfin tuna ( Thunnus albacares ). Journal Of Applied Microbiology, 124(2), 559-571. Publisher's official version : https://doi.org/10.1111/jam.13663 , Open Access version : https://archimer.ifremer.fr/doc/00414/52557/