Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology
| Type | Article | ||||||||||||
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| Date | 2017-12 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Dribek Mohamed1, 3, Rinnert Emmanuel 1, Colas Florent1, Crassous Marie-Pierre1, Thioune Nene2, David Catalina2, 4, de La Chapelle Marc2, Compere Chantal1 |
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| Affiliation(s) | 1 : IFREMER, Lab Detect Capteurs & Mesures, CS10070, F-29280 Plouzane, France. 2 : Univ Paris 13, UFR SMBH, Lab CSPBAT FRE 3043, 74 Rue Marcel Cachin, F-93017 Bobigny, France. 3 : Univ Francois Rabelais Tours, UFR Sci Pharmaceut, EA Nanomedicaments & Nanosondes 6295, 31 Ave Monge, F-37200 Tours, France. 4 : Horiba Sci, 231 Rue Lille, F-59650 Villeneuve Dascq, France. |
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| Source | Environmental Science And Pollution Research (0944-1344) (Springer Heidelberg), 2017-12 , Vol. 24 , N. 35 , P. 27070-27076 | ||||||||||||
| DOI | 10.1007/s11356-014-3384-8 | ||||||||||||
| WOS© Times Cited | 17 | ||||||||||||
| Note | New methods and methodologies for the pollutant detection | ||||||||||||
| Keyword(s) | Biosensor, Surface-enhanced Raman scattering, Polycyclic aromatic hydrocarbons, Benzo[a]pyrene antibody, Organometallic nanoprobe | ||||||||||||
| Abstract | We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH2-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with 6-formylbenzo[a]pyrene. So, this SERS detection involves four steps: (i) the nanoprobes are incubated with the sample; (ii) a drop of the mixture is then put onto the substrate; (iii) the surface is rinsed; and (iv) the surface is analyzed by Raman spectroscopy. To synthesize the nanoprobes, firstly, we prepared GNPs according to Frens' method. Then, GNPs were spontaneously labeled by the DSNB Raman reporter, thanks to a strong gold-sulfur interaction. Thereafter, BaP antibodies were cross-linked to the DSNB labeled GNPs by reaction of proteins primary amino groups with N-hydroxyl succinimide (NHS). Before use in SERS detection, their activity was controlled by surface plasmon resonance technique. The present method allows us to detect BaP at trace concentration (2 nmol/L). The results demonstrate that the proposed method has a great potential for application in the monitoring of seawater. | ||||||||||||
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