The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect

Type Article
Date 2018-02
Language English
Author(s) Delisle Lizenn1, 2, Fuhrmann Marine1, Quere Claudie1, Pauletto Marianna3, Pichereau Vianney4, Pernet FabriceORCID1, Corporeau Charlotte1
Affiliation(s) 1 : IFREMER, CNRS, Lab Sci Environm Marin LEMAR, UBO,IRD,UMR 6539, F-29280 Plouzane, France.
2 : IFREMER, Ctr Ifremer Bretagne, Unite Physiol Fonct Organ Marins PFOM, LPI, 1625 Route St Anne,CS 10070, F-29280 Plouzane, France.
3 : Univ Padua, Dept Comparat Biomed & Food Sci, Viale Univ 16, I-35020 Padua, Italy.
4 : Univ Bretagne Occidentale, CNRS, IFREMER, Lab Sci Environm Marin LEMAR,UBO,IRD,UMR 6539, F-29280 Plouzane, France.
Source Marine Biotechnology (1436-2228) (Springer), 2018-02 , Vol. 20 , N. 1 , P. 87-97
DOI 10.1007/s10126-017-9789-x
WOS© Times Cited 3
Keyword(s) Voltage-dependent anion channel, Warburg effect, Crassostrea gigas, Ostreid herpes virus
Abstract

Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment.

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Delisle Lizenn, Fuhrmann Marine, Quere Claudie, Pauletto Marianna, Pichereau Vianney, Pernet Fabrice, Corporeau Charlotte (2018). The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect. Marine Biotechnology, 20(1), 87-97. Publisher's official version : https://doi.org/10.1007/s10126-017-9789-x , Open Access version : https://archimer.ifremer.fr/doc/00420/53129/