Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment

Type Article
Date 2018-05
Language English
Author(s) Zykwinska AgataORCID1, Berre Ludivine Tripon-Le1, Sinquin CorinneORCID1, Ropartz David2, Rogniaux Helene2, Colliec-Jouault SylviaORCID1, Delbarre-Ladrat ChristineORCID1
Affiliation(s) 1 : IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol, F-44311 Nantes, France.
2 : INRA, Biopolymeres Interact Assemblages UR1268, F-44300 Nantes, France.
Source Carbohydrate Polymers (0144-8617) (Elsevier Sci Ltd), 2018-05 , Vol. 188 , P. 101-107
DOI 10.1016/j.carbpol.2018.01.086
WOS© Times Cited 7
Keyword(s) Exopolysaccharide, Enzyme, Depolymerization, Oligosaccharide, Mass spectrometry
Abstract

Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened for their depolymerizing activities, however none of them was able to degrade the polysaccharide. Enzymatic assays performed with A. infernus protein extracts have shown that bacterium produces by itself endogenous enzymes able to depolymerize its own EPS. The oligosaccharides released by the enzymes were analyzed and their structures allowed to assess that the protein extract contains several depolymerizing activities.

Full Text
File Pages Size Access
Author's final draft 28 588 KB Open access
5 212 KB Access on demand
7 488 KB Access on demand
Top of the page

How to cite 

Zykwinska Agata, Berre Ludivine Tripon-Le, Sinquin Corinne, Ropartz David, Rogniaux Helene, Colliec-Jouault Sylvia, Delbarre-Ladrat Christine (2018). Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment. Carbohydrate Polymers, 188, 101-107. Publisher's official version : https://doi.org/10.1016/j.carbpol.2018.01.086 , Open Access version : https://archimer.ifremer.fr/doc/00423/53458/