A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae , Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

Type Article
Date 2019-01
Language English
Author(s) Hartnell R. E.1, Stockley L.1, Keay W.1, Rosec J. -P.2, Hervio-Heath Dominique3, Van Den Berg H.4, Leoni F.5, Ottaviani D.5, Henigman U.6, Denayer S.7, Serbruyns B.8, Georgsson F.9, Krumova-Valcheva G.10, Gyurova E.10, Blanco C.11, Copin S.12, Strauch E.13, Wieczorek K.14, 15, Lopatek M.15, Britova A.16, Hardouin G.17, Lombard B.18, Veld P. In'T19, Leclercq A., Baker-Austin C.1
Affiliation(s) 1 : European Union Reference Lab Monitoring Bacteriol, Ctr Environm Fisheries & Aquaculture Sci Cefas, Barrack Rd, Nothe DT4 8UB, Weymouth, England.
2 : Minist Econ & Finances, Serv Conunun Lab, Montpellier, France.
3 : Ctr Ifremer Brest, LNR Microbiol Coquillages, Lab Microbiol EMP MIC, BP 70, F-29280 Plouzane, France.
4 : Natl Inst Publ Hlth & Environm RIVM, POB 1,Antonie Leeuwenhoeklaan 9, NL-3720 BA Bilthoven, Netherlands.
5 : Ist Zooprofilatt Sperimentale Umbria Marche, Sez Ancona, Ancona, Italy.
6 : Fac Vet, Inst Food Hyg & Bromatol, Gerbiceva 60, SI-1000 Ljubljana, Slovenia.
7 : Sci Inst Publ Hlth, Sci Serv Food Borne Pathogens, Juliette Wytsmanstr 14, B-1050 Brussels, Belgium.
8 : Chemiphar Nv, Lieven Bauwensstr 4, B-8200 Brugge, Belgium.
9 : Iceland Food & Biotech R&D, Vinlandsleio 12, Reykjavik 113, Iceland.
10 : Food Safety Lab, 15 Pencho Slaveikov Blvd, Sofia 1606, Bulgaria.
11 : Agencia Espanola Sguridad Alimentaria, Ctr Nacl Alimentat, Serv Microbiol Alimentaria, Carretera Majadahonda Pozuelo,Km 5 2, Madrid 28220, Spain.
12 : ANSES, Lab Prod Peche Boulognesurmer, Blvd Bassin Napoleon, F-62200 Boulogne Sur Mer, France.
13 : German Fed Inst Risk Assessment, Bundesinst Risikobewertung BfR, D-112277 Berlin, Germany.
14 : Dept Hyg Food Anim Origin, Dept Food & Environm Virol, Partyzantow 57, PL-24100 Pulawy, Poland.
15 : State Vet & Food Inst, Janoskova 1611-58, Dolny Kubin 02601, Slovakia.
16 : Assoc Frangaise Normalisat, St Denis, Reunion, France.
17 : Agence Natl Secur Sanit Alimentat Environm & Trav, Maisons Alfort, France.
18 : Netherlands Food & Consumer Prod Safety Author, Utrecht, Netherlands.
19 : Inst Pasteur, Paris, France.
Source International Journal Of Food Microbiology (0168-1605) (Elsevier Science Bv), 2019-01 , Vol. 288 , P. 58-65
DOI 10.1016/j.ijfoodmicro.2018.02.008
WOS© Times Cited 3
Keyword(s) Vibrios, Seafood, Prawns, Oysters, Biochemical methods, Real-time PCR, Conventional PCR

Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus) was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also considered simplification of the biochemical confirmation steps. In addition, because of advances in molecular based methods for identification of human pathogenic Vibrio spp. classical and real time PCR options were also included within the scope of the validation. These considerations formed the basis of a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp.. To achieve this aim, an international validation study involving 13 laboratories from 9 countries in Europe was conducted in 2013. The results of this validation have enabled integration of the two existing technical specifications targeting the detection of the major foodborne Vibrio spp., simplification of the suite of recommended biochemical identification tests and the introduction of molecular procedures that provide both species level identification and discrimination of putatively pathogenic strains of V. parahaemolyticus by the determination of the presence of theromostable direct and direct related haemolysins. The method performance characteristics generated in this have been included in revised international standard, ISO 21872:2017, published in July 2017.

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Hartnell R. E., Stockley L., Keay W., Rosec J. -P., Hervio-Heath Dominique, Van Den Berg H., Leoni F., Ottaviani D., Henigman U., Denayer S., Serbruyns B., Georgsson F., Krumova-Valcheva G., Gyurova E., Blanco C., Copin S., Strauch E., Wieczorek K., Lopatek M., Britova A., Hardouin G., Lombard B., Veld P. In'T, Leclercq A., Baker-Austin C. (2019). A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae , Vibrio parahaemolyticus and Vibrio vulnificus in seafoods. International Journal Of Food Microbiology, 288, 58-65. Publisher's official version : https://doi.org/10.1016/j.ijfoodmicro.2018.02.008 , Open Access version : https://archimer.ifremer.fr/doc/00425/53680/