Metabolism of the Pacific oyster, Crassostrea gigas , is influenced by salinity and modulates survival to the Ostreid herpesvirus OsHV-1

Type Article
Date 2018-02
Language English
Author(s) Fuhrmann Marine1, Delisle Lizenn1, Petton Bruno2, Corporeau Charlotte1, Pernet FabriceORCID1
Affiliation(s) 1 : Technopole Brest Iroise, IFREMER, UBO CNRS IRD, LEMAR UMR 6539, F-29280 Plouzane, France.
2 : IFREMER, UBO CNRS IRD, LEMAR UMR 6539, F-29840 Argenton, France.
Source Biology Open (2046-6390) (Company Of Biologists Ltd), 2018-02 , Vol. 7 , N. 2 , P. bio028134 (10p.)
DOI 10.1242/bio.028134
WOS© Times Cited 2
Keyword(s) Bivalve, Disease, Environment, Metabolism, Mortality risk, Salinity
Abstract

The Pacific oyster, Crassostrea gigas, is an osmoconforming bivalve exposed to wide salinity fluctuations. The physiological mechanisms used by oysters to cope with salinity stress are energy demanding and may impair other processes, such as defense against pathogens. This oyster species has been experiencing recurrent mortality events caused by the Ostreid herpesvirus 1 (OsHV-1). The objectives of this study were to investigate the effect of salinity (10, 15, 25 and 35‰) on energetic reserves, key enzyme activities and membrane fatty acids, and to identify the metabolic risk factors related to OsHV-1-induced mortality of oysters. Acclimation to low salinity led to increased water content, protein level, and energetic reserves (carbohydrates and triglycerides) of oysters. The latter was consistent with lower activity of hexokinase, the first enzyme involved in glycolysis, up-regulation of AMP-activated protein kinase, a major regulator of cellular energy metabolism, and lower activity of catalase, an antioxidant enzyme involved in management of reactive oxygen species. Acclimation to salinity also involved a major remodeling of membrane fatty acids. Particularly, 20:4n-6 decreased linearly with decreasing salinity, likely reflecting its mobilization for prostaglandin synthesis in oysters. The survival of oysters exposed to OsHV-1 varied from 43% to 96% according to salinity ( Fuhrmann et al., 2016). Risk analyses showed that activity of superoxide dismutase and levels of proteins, carbohydrates, and triglycerides were associated with a reduced risk of death. Therefore, animals with a higher antioxidant activity and a better physiological condition seemed less susceptible to OsHV-1.

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Fuhrmann Marine, Delisle Lizenn, Petton Bruno, Corporeau Charlotte, Pernet Fabrice (2018). Metabolism of the Pacific oyster, Crassostrea gigas , is influenced by salinity and modulates survival to the Ostreid herpesvirus OsHV-1. Biology Open, 7(2), bio028134 (10p.). Publisher's official version : https://doi.org/10.1242/bio.028134 , Open Access version : https://archimer.ifremer.fr/doc/00427/53886/