Specific enrichment of hyperthermophilic electroactive Archaea from deep-sea hydrothermal vent on electrically conductive support.
|Author(s)||Pillot Guillaume1, Frouin Eleonore1, Pasero Emilie1, Godfroy Anne2, Combet-Blanc Yannick1, Davidson Sylvain1, Liebgott Pierre-Pol1|
|Affiliation(s)||1 : Univ Toulon & Var, Aix Marseille Univ, CNRS, IRD,MIO UM 110, Marseille, France.
2 : Univ Bretagne Occidentale, CNRS, IFREMER,UM6197, Ctr Brest CS10070,Lab Microbiol Environm Extremes, Plouzane, France.
|Source||Bioresource Technology (0960-8524) (Elsevier Sci Ltd), 2018-07 , Vol. 259 , P. 304-311|
|WOS© Times Cited||11|
|Keyword(s)||Hyperthermophilic exoelectrogens, Deep-sea hydrothermal vent, Microbial electrolysis cell, External electron transfer|
While more and more investigations are done to study hyperthermophilic exoelectrogenic communities from environments, none have been performed yet on deep-sea hydrothermal vent. Samples of black smoker chimney from Rainbow site on the Atlantic mid-oceanic ridge have been harvested for enriching exoelectrogens in microbial electrolysis cells under hyperthermophilic (80 °C) condition. Two enrichments were performed in a BioElectrochemical System specially designed: one from direct inoculation of crushed chimney and the other one from inoculation of a pre-cultivation on iron (III) oxide. In both experiments, a current production was observed from 2.4 A/m2 to 5.8 A/m2 with a set anode potential of -0.110 V vs Ag/AgCl. Taxonomic affiliation of the exoelectrogen communities obtained on the electrode exhibited a specific enrichment of Archaea belonging to Thermococcales and Archeoglobales orders, even when both inocula were dominated by Bacteria.