Survival, growth and fatty acid composition of Ostrea edulis larvae (L.) fed four different single species, microalgal diets, Tisochrysis lutea (T), Chaetoceros neogracile (Cg), Skeletonema marinoi (Sm) or Tetraselmis suecica (Ts) from broodstock to pre-settlement, were studied. Lower larval growth (5.5m to 6.5m/d) was recorded in progeny continuously fed single S.marinoi or T.suecica, whereas good growth was achieved with single T.lutea (7.8m/d). Larvae, originated from broodstock receiving Sm or Ts, exhibited growth compensation when fed a bispecific balanced diet (TCg). This did not occur when broodstock and larvae were fed Cg or T, for which single or mixed diets led to similar larval growth. Furthermore, survival was high (>90%) regardless of microalgal diet, except for larvae fed from broodstock to pre-settlement T (53%) or Ts (2%). There were significant differences in 20:5 (n-3) and 22: 6 (n-3) contents in polar and neutral fractions of O.edulis expelled larvae dependent on broodstock diet, as well as throughout larval development, but no clear trend was apparent when comparing fatty acid (FA) relative composition of both fractions of O.edulis larvae fed different diets at release or prior to settlement. In contrast, such correlation occurred when FA was expressed in absolute content but exclusively for larvae-fed single diets and was particularly noticeable between 22: 6 (n-3) and growth and survival. In the present work, broodstock nutritional deficiencies have been revealed in O.edulis progeny, compensated thereafter by feeding the larvae a mixed diet, and in this balanced condition, no obvious relation with larval development indicators was found with main fatty acid contents.