Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey

Type Article
Date 2018-05
Language English
Author(s) Garcia-Portela Maria1, Reguera Beatriz1, Sibat ManoellaORCID2, Altenburger AndreasORCID3, Rodriguez Francisco1, Hess PhilippORCID2
Affiliation(s) 1 : IEO, Oceanog Ctr Vigo, Subida Radio Faro 50, Vigo 36390, Spain.
2 : IFREMER, Phycotoxins Lab, Rue Ile Yeu,BP 21105, F-44311 Nantes, France.
3 : Univ Copenhagen, Nat Hist Museum Denmark, Oster Voldgade 5-7, DK-1350 Copenhagen, Denmark.
Source Marine Drugs (1660-3397) (Mdpi), 2018-05 , Vol. 16 , N. 5 , P. 143 (25p.)
DOI 10.3390/md16050143
WOS© Times Cited 6
Keyword(s) Dinophysis physiology, lipophilic toxins, non-targeted analysis, metabolomics
Abstract

Photosynthetic species of the genus Dinophysis are obligate mixotrophs with temporary plastids (kleptoplastids) that are acquired from the ciliate Mesodinium rubrum, which feeds on cryptophytes of the Teleaulax-Plagioselmis-Geminigera clade. A metabolomic study of the three-species food chain Dinophysis-Mesodinium-Teleaulax was carried out using mass spectrometric analysis of extracts of batch-cultured cells of each level of that food chain. The main goal was to compare the metabolomic expression of Galician strains of Dinophysis acuminata and D. acuta that were subjected to different feeding regimes (well-fed and prey-limited) and feeding on two Mesodinium (Spanish and Danish) strains. Both Dinophysis species were able to grow while feeding on both Mesodinium strains, although differences in growth rates were observed. Toxin and metabolomic profiles of the two Dinophysis species were significantly different, and also varied between different feeding regimes and different prey organisms. Furthermore, significantly different metabolomes were expressed by a strain of D. acuminata that was feeding on different strains of the ciliate Mesodinium rubrum. Both species-specific metabolites and those common to D. acuminata and D. acuta were tentatively identified by screening of METLIN and Marine Natural Products Dictionary databases. This first metabolomic study applied to Dinophysis acuminata and D.acuta in culture establishes a basis for the chemical inventory of these species

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Garcia-Portela Maria, Reguera Beatriz, Sibat Manoella, Altenburger Andreas, Rodriguez Francisco, Hess Philipp (2018). Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey. Marine Drugs, 16(5), 143 (25p.). Publisher's official version : https://doi.org/10.3390/md16050143 , Open Access version : https://archimer.ifremer.fr/doc/00439/55077/