Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles

Type Article
Date 2018-10
Language English
Author(s) Gonzalez-Fernandez CarmenORCID1, Tallec Kevin2, Le Goic Nelly1, Lambert Christophe1, Soudant Philippe1, Huvet ArnaudORCID2, Suquet Marc2, Berchel Mathieu3, Paul-Pont Ika1
Affiliation(s) 1 : IUEM, Lab Sci Environm Marin LEMAR, UMR CNRS 6539, UBO,IRD,IFREMER, Rue Dumont Urville, F-29280 Plouzane, France.
2 : IFREMER, Lab Sci Environm Marin LEMAR, CS 10070, F-29280 Plouzane, France.
3 : Univ Bretagne Occidentale, UFR Sci, CEMCA, UMR CNRS 6521,IBSAM, 6 Ave Victor Le Gorgeu, F-29238 Brest, France.
Source Chemosphere (0045-6535) (Pergamon-elsevier Science Ltd), 2018-10 , Vol. 208 , P. 764-772
DOI 10.1016/j.chemosphere.2018.06.039
WOS© Times Cited 23
Keyword(s) Nanoplastics, Oysters, Gametes, Cellular responses
Abstract

While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the adhesion of NPs to oyster spermatozoa and oocytes as suggested by the increase of relative cell size and complexity measured by flow-cytometry and confirmed by microscopy observations. A significant increase of ROS production was observed in sperm cells upon exposure to 100 mg L−1 PS-COOH, but was not observed with PS-NH2, suggesting a differential effect according to the NP-associated functional group. Altogether, these results demonstrate that the effects of NPs occur rapidly, are complex and are possibly associated with the cellular eco-corona, which could modify NPs behaviour and toxicity.

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Gonzalez-Fernandez Carmen, Tallec Kevin, Le Goic Nelly, Lambert Christophe, Soudant Philippe, Huvet Arnaud, Suquet Marc, Berchel Mathieu, Paul-Pont Ika (2018). Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles. Chemosphere, 208, 764-772. Publisher's official version : https://doi.org/10.1016/j.chemosphere.2018.06.039 , Open Access version : https://archimer.ifremer.fr/doc/00443/55459/