Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities

Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or time of filtration could be modulated following the cross-flow velocity. Polysaccharides were concentrated 6.3 to 10.4 times in such a way that the final sugars concentration reached 1.74 to 2.26 g.L-1. Rheological behavior of filtered solutions changed following the concentration progress. More than 80 % (w/w) of polysaccharides were recovered while 49 % and 99 % of proteins and salts were removed respectively. The filtrations allowed reaching a final monosaccharide content of dry matter equal to 48.9 % against 0.6 % (w/w) initially.

Keyword(s)

Membrane filtration, Exopolysaccharides, Microalga, Concentration, Diafiltration

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Appendix A. Method for the determination of gel-polarization model parameters The sugar concentration profile in the vicinity of the membrane may be represented by the Fig. A1.
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Balti Rafik, Le Balc'h Romain, Brodu Nicolas, Gilbert Marthe, Le Gouic Benjamin, Le Gall Sophie, Sinquin Corinne, Masse Anthony (2018). Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities. Process Biochemistry. 74. 175-184. https://doi.org/10.1016/j.procbio.2018.06.021, https://archimer.ifremer.fr/doc/00445/55694/

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