Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence

Type Article
Date 2018-07
Language English
Author(s) Saraoui Taous1, 2, Leroi FrancoiseORCID1, Chevalier FrederiqueORCID1, Cappelier Jean-Michel2, Passerini DelphineORCID1, Pilet Marie-France2
Affiliation(s) 1 : Inst Francais Rech Exploitat Mer Ifremer, Lab Ecosyst Microbiens & Mol Marines Biotechnol E, Nantes, France.
2 : Ecole Natl Vet Agroalimentaire & Alimentat Nantes, INRA, SECALIM UMR1014, Nantes, France.
Source Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2018-07 , Vol. 9 , N. 1564 , P. 10p.
DOI 10.3389/fmicb.2018.01564
WOS© Times Cited 19
Keyword(s) biopreservation, Lactococcus piscium, Listeria monocytogenes, co-culture, cell ratio, scanning electron microscopy, virulence

Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached its maximum population density, whatever the initial concentration of the protective bacteria. Viewed by scanning electron microscopy, L. monocytogenes cell shape and surface appeared modified in co-culture with L. piscium CNCM I-4031. Lastly, L. monocytogenes virulence, evaluated by a plaque-forming assay on the HT-29 cell line, was reduced after cell pre-treatment by the protective bacteria. In conclusion, the bioprotective effect of L. piscium toward L. monocytogenes growth and virulence was demonstrated, and a hypothesis for the inhibition mechanism is put forward.

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Saraoui Taous, Leroi Francoise, Chevalier Frederique, Cappelier Jean-Michel, Passerini Delphine, Pilet Marie-France (2018). Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence. Frontiers In Microbiology, 9(1564), 10p. Publisher's official version : , Open Access version :