Pre-purification by membrane filtration of paralytic shellfish toxins from Alexandrium minutum dinoflagellate
|Author(s)||Balti Rafik1, 2, Brodu Nicolas1, 3, Zhang Jiaxuan1, Amzil Zouher4, Drouin Delphine1, Sechet Veronique4, Masse Anthony1|
|Affiliation(s)||1 : Univ Nantes, LUNAM, GEPEA, UMR CNRS 6144, 37 Bd Univ,BP 406, F-44602 St Nazaire, France.
2 : Univ Jendouba, Higher Inst Biotechnol Beja, Unite Physiol Fonct & Valorisat Bioressources UR1, PB 382,Habib Bourguiba Ave, Beja 9000, Tunisia.
3 : NormandieUniv, UNIROUEN, INSA Rouen, LSPC, F-76000 Rouen, France.
4 : IFREMER, Lab Phycotoxines, Ctr Nantes, BP 21105, F-44311 Nantes, France.
|Source||Separation And Purification Technology (1383-5866) (Elsevier Science Bv), 2019-02 , Vol. 210 , P. 152-158|
|WOS© Times Cited||1|
|Keyword(s)||Paralytic shellfish poisoning, Nanofiltration, Membrane, Alexandrium minutum, Purification|
The Paralytic shellfish neurotoxins (PST) are of increasing interest for biomedical applications. The chemical synthesis is often complex and expensive that’s why the purification by membrane filtration of PST from Alexandrium minutum dinoflagellate was investigated. Disrupted micro-alga cells by ultrasonic treatment were diafiltred to let pass toxins through an ultrafiltration membrane. Then, the mean permeate was concentrated and diafiltrated by nanofiltration. Mean permeate fluxes equal to 187, 135 and 135 L.h–1.m-2 were obtained during the first diafiltration, the concentration step and the final diafiltration respectively. Up to 57 % (mol/mol) and 78 % (mol/mol) of organic matters and salts were removed respectively. Divalent ions were sparsely removed contrary to monovalent ones. C1 and C2 toxins were successfully purified since more than 75 % (mol/mol) were recovered. However, only 27 to 50 % (mol/mol) of GTX2, GTX3 and STX were recovered.