Detection of new pathways involved in the acceptance and the utilisation of a plant-based diet in isogenic lines of rainbow trout fry

Type Article
Date 2018-07
Language English
Author(s) Callet Therese1, 2, Dupont-Nivet Mathilde1, Cluzeaud Marianne2, Jaffrezic Florence1, Laloe Denis1, Kerneis Thierry3, Labbe Laurent3, Quillet Edwige1, Geurden Inge2, Mazurais DavidORCID4, Skiba-Cassy Sandrine2, Medale Francoise2
Affiliation(s) 1 : Univ Paris Saclay, AgroParisTech, INRA, UMR GABI, Jouy En Josas, France.
2 : INRA, UMR NuMeA, St Pee Sur Niyelle, France.
3 : INRA, PEIMA, Sizun, France.
4 : IFREMER, Brest, France.
Source Plos One (1932-6203) (Public Library Science), 2018-07 , Vol. 13 , N. 7 , P. e0201462 (22p.)
DOI 10.1371/journal.pone.0201462
WOS© Times Cited 4
Abstract

o meet the growing demand of fish feed for aquaculture, an increasing proportion of marine ingredients are being replaced by blends of plant products. However, the total replacement of marine ingredients in salmonid diets impairs fish performance. This is particularly true during the early fry stage and this stage is therefore considered of particular importance. In rainbow trout (RBT), the existence of a genetic variability to survive and grow with plant-based diets devoid of marine ingredients has now been proved, but the mechanisms behind are little studied especially at early stage. To investigate these, we analysed the whole transcriptome of three isogenic lines of RBT fry, which have similar growth when fed a marine resources-based diet (M diet) but which highly differ in their responses to a plant-based diet (V diet). Analysis of transcriptomes profiles revealed 1740, 1834 and 246 probes differentially expressed among the three genotypes when fed the V diet. The use of these lines led to the discovery of potential molecular markers linked to plant-based diet utilisation, some of them belonging to new pathways, never described before. An important number of genes was related to immunity, but further investigations are needed to better understand the difference between the genotypes in their immune status response to V diet exposure. Finally, differences in expression of genes related to feed intake and sensory perception among genotypes suggested that the mechanisms underlying the differences in growth on plant-based diet are closely linked to diet acceptance. Research on plants components affecting feed intake should be thus further explored

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Publisher's official version 22 1 MB Open access
S1 File. Sequence of primers used for gene expression analysis by real-time PCR. 7 KB Open access
S2 File. Fold changes and p-value associated with each gene found differentially expressed among the three genotypes, identified as potential molecular markers linked to plant-based diets utilisation. 223 KB Open access
S3 File. GO terms found significantly enriched (Cut-off: p-value<0.01; Benjamini<0.1). 6 KB Open access
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How to cite 

Callet Therese, Dupont-Nivet Mathilde, Cluzeaud Marianne, Jaffrezic Florence, Laloe Denis, Kerneis Thierry, Labbe Laurent, Quillet Edwige, Geurden Inge, Mazurais David, Skiba-Cassy Sandrine, Medale Francoise (2018). Detection of new pathways involved in the acceptance and the utilisation of a plant-based diet in isogenic lines of rainbow trout fry. Plos One, 13(7), e0201462 (22p.). Publisher's official version : https://doi.org/10.1371/journal.pone.0201462 , Open Access version : https://archimer.ifremer.fr/doc/00452/56371/