Crystal Structure of ChrR-A Quinone Reductase with the Capacity to Reduce Chromate
| Type | Article |
|---|---|
| Date | 2012-04 |
| Language | English |
| Author(s) | Eswaramoorthy Subramaniam1, Poulain Sebastien2, 3, 4, 5, Hienerwadel Rainer4, 6, 7, Bremond Nicolas3, 4, 5, Sylvester Matthew D.2, Zhang Yian-Biao1, Berthomieu Catherine 3, 4, 5, Van Der Lelie Daniel8, Matin A.2 |
| Affiliation(s) | 1 : Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. 2 : Stanford Univ, Dept Microbiol & Immunol, Stanford, CA 94305 USA. 3 : CEA, DSV IBEB, Lab Interact Prot Met, St Paul Les Durance, France. 4 : CNRS, UMR Biol Vegetale & Microbiol Environm, St Paul Les Durance, France. 5 : Univ Aix Marseille, St Paul Les Durance, France. 6 : Univ Aix Marseille, Lab Genet & Biophys Plantes, Marseille, France. 7 : CEA, DSV IBEB, Marseille, France. 8 : RTI Int, Discovery & Analyt Sci, Res Triangle Pk, NC USA. |
| Source | Plos One (1932-6203) (Public Library Science), 2012-04 , Vol. 7 , N. 4 , P. e36017 (7p.) |
| DOI | 10.1371/journal.pone.0036017 |
| WOS© Times Cited | 53 |
| Abstract | The Escherichia coli ChrR enzyme is an obligatory two-electron quinone reductase that has many applications, such as in chromate bioremediation. Its crystal structure, solved at 2.2 angstrom resolution, shows that it belongs to the flavodoxin superfamily in which flavin mononucleotide (FMN) is firmly anchored to the protein. ChrR crystallized as a tetramer, and size exclusion chromatography showed that this is the oligomeric form that catalyzes chromate reduction. Within the tetramer, the dimers interact by a pair of two hydrogen bond networks, each involving Tyr128 and Glu146 of one dimer and Arg125 and Tyr85 of the other; the latter extends to one of the redox FMN cofactors. Changes in each of these amino acids enhanced chromate reductase activity of the enzyme, showing that this network is centrally involved in chromate reduction. |
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