Age- and quality-dependent DNA methylation correlate with melanin-based coloration in a wild bird

Type Article
Date 2018-07
Language English
Author(s) Soulsbury Carl D.1, Lipponen Anssi2, 3, Wood Kristie4, Mein Charles A.4, Hoffman Joseph I.5, Lebigre ChristopheORCID6
Affiliation(s) 1 : Univ Lincoln, Sch Life Sci, Lincoln, England.
2 : Univ Jyvaskyla, Dept Biol & Environm Sci, Jyvaskyla, Finland.
3 : Univ Eastern Finland, AI Virtanen Inst Mol Sci, Kuopio, Finland.
4 : Queen Mary Univ London, Barts & London Sch Med & Dent, Genome Ctr, London, England.
5 : Univ Bielefeld, Dept Anim Behav, Bielefeld, Germany.
6 : French Res Inst Exploitat Sea, Plouzane, France.
Source Ecology And Evolution (2045-7758) (Wiley), 2018-07 , Vol. 8 , N. 13 , P. 6547-6557
DOI 10.1002/ece3.4132
WOS© Times Cited 4
Keyword(s) AgRP, coloration, epigenetics, heterozygosity, melanin, sexual ornament

Secondary sexual trait expression can be influenced by fixed individual factors (such as genetic quality) as well as by dynamic factors (such as age and environmentally induced gene expression) that may be associated with variation in condition or quality. In particular, melanin-based traits are known to relate to condition and there is a well-characterized genetic pathway underpinning their expression. However, the mechanisms linking variable trait expression to genetic quality remain unclear. One plausible mechanism is that genetic quality could influence trait expression via differential methylation and differential gene expression. We therefore conducted a pilot study examining DNA methylation at a candidate gene (agouti-related neuropeptide: AgRP) in the black grouse Lyrurus tetrix. We specifically tested whether CpG methylation covaries with age and multilocus heterozygosity (a proxy of genetic quality) and from there whether the expression of a melanin-based ornament (ultraviolet-blue chroma) correlates with DNA methylation. Consistent with expectations, we found clear evidence for age- and heterozygosity-specific patterns of DNA methylation, with two CpG sites showing the greatest DNA methylation in highly heterozygous males at their peak age of reproduction. Furthermore, DNA methylation at three CpG sites was significantly positively correlated with ultraviolet-blue chroma. Ours is the first study to our knowledge to document age- and quality-dependent variation in DNA methylation and to show that dynamic sexual trait expression across the lifespan of an organism is associated with patterns of DNA methylation. Although we cannot demonstrate causality, our work provides empirical support for a mechanism that could potentially link key individual factors to variation in sexual trait expression in a wild vertebrate.

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Soulsbury Carl D., Lipponen Anssi, Wood Kristie, Mein Charles A., Hoffman Joseph I., Lebigre Christophe (2018). Age- and quality-dependent DNA methylation correlate with melanin-based coloration in a wild bird. Ecology And Evolution, 8(13), 6547-6557. Publisher's official version : , Open Access version :