Molecular and cellular characterization of apoptosis in flat oyster a key mechanisms at the heart of host-parasite interactions

Type Article
Date 2018-08
Language English
Author(s) Gervais Ophelie1, Renault Tristan2, Arzul IsabelleORCID1
Affiliation(s) 1 : Ifremer, Stn La Tremblade, RBE LGPMM SG2M, Ave Mus Loup, F-17390 La Tremblade, France.
2 : Ifremer, Ctr Nantes, RBE, Rue Ile dYeu, Nantes, France.
Source Scientific Reports (2045-2322) (Nature Publishing Group), 2018-08 , Vol. 8 , N. 1 , P. 12494 (12p.)
DOI 10.1038/s41598-018-29776-x
WOS© Times Cited 11
Abstract

Bonamia ostreae has been associated with the decline of flat oyster Ostrea edulis populations in some European countries. This obligatory intracellular parasite persists and multiplies into hemocytes. Previous in vitro experiments showed that apoptosis is activated in hemocytes between 1 h and 4 h of contact with the parasite. The flat oyster uses the apoptosis pathway to defend against B. ostreae. However, the parasite might be also able to modulate this response in order to survive in its host. In order to investigate this hypothesis the apoptotic response of the host was evaluated using flow cytometry, transmission electron microscopy and by measuring the response of genes involved in the apoptotic pathway after 4 h. In parallel, the parasite response was investigated by measuring the expression of B. ostreae genes involved in different biological functions including cell cycle and cell death. Obtained results allow describing molecular apoptotic pathways in O. edulis and confirm that apoptosis is early activated in hemocytes after a contact with B. ostreae. Interestingly, at cellular and molecular levels this process appeared downregulated after 44 h of contact. Concurrently, parasite gene expression appeared reduced suggesting that the parasite could inhibit its own metabolism to escape the immune response.

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