In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement

Type Article
Date 2019-01
Language English
Author(s) Foulon Valentin1, Boudry PierreORCID2, Artigaud Sebastien1, Guérard Fabienne1, Hellio Claire1
Affiliation(s) 1 : Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 CNRS/UBO/IRD/Ifremer, Institut Universitaire Européen de la Mer, Technopole Brest-Iroise, Rue Dumont d’Urville, 29280 Plouzané, France
2 : Ifremer, Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 CNRS/UBO/IRD/Ifremer, Centre Bretagne, 29280 Plouzané, France
Source International Journal Of Molecular Sciences (1422-0067) (MDPI AG), 2019-01 , Vol. 20 , N. 1 , P. 197 (16p.)
DOI 10.3390/ijms20010197
Note This article belongs to the Special Issue Transcriptional Regulation: Molecules, Involved Mechanisms and Misregulation
Keyword(s) Crassostrea gigas, Pacific oyster, pediveliger larvae, bioadhesive, transcriptome
Abstract

Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae.

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Foulon Valentin, Boudry Pierre, Artigaud Sebastien, Guérard Fabienne, Hellio Claire (2019). In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement. International Journal Of Molecular Sciences, 20(1), 197 (16p.). Publisher's official version : https://doi.org/10.3390/ijms20010197 , Open Access version : https://archimer.ifremer.fr/doc/00475/58677/