Whole transcriptome sequencing and biomineralization gene architecture associated with cultured pearl quality traits in the pearl oyster, Pinctada margaritifera

Type Article
Date 2019-02
Language English
Author(s) Le Luyer JeremyORCID1, Auffret Pauline1, Quillien Virgile1, Leclerc Nicolas1, Reisser CelineORCID1, Vidal-Dupiol Jeremie1, 2, Ky Chin-Long1
Affiliation(s) 1 : Ctr Ifremer Pacifique, Labex Corail, EIO, IFREMER,UMR 241, BP 49, F-98719 Tahiti, French Polynesi, France.
2 : Univ Montpellier, IFREMER, UMR Interact Hotes Pathogenes Environm 5244, Pl Eugene Bataillon CC 80, F-34095 Montpellier, France.
Source Bmc Genomics (1471-2164) (Bmc), 2019-02 , Vol. 20 , P. -
DOI 10.1186/s12864-019-5443-5
WOS© Times Cited 5
Keyword(s) Pinctada margaritifera, Biomineralization, Pearl quality, Pearl sac, RNA-seq
Abstract

Background

Cultured pearls are unique gems produced by living organisms, mainly molluscs of the  Pinctada genus, through the biomineralization properties of pearl sac tissue.  Improvement of P. margaritifera pearl quality is one of the biggest challenges that  Polynesian research has faced to date. To achieve this goal, a better understanding of the  complex mechanisms related to nacre and pearl formation is essential and can now be  approached through the use of massive parallel sequencing technologies. The aim of this  study was to use RNA-seq to compare whole transcriptome expression of pearl sacs that  had producing pearls with high and low quality. For this purpose, a comprehensive  reference transcriptome of P. margaritifera was built based on multi-tissue sampling  (mantle, gonad, whole animal), including different living stages (juvenile, adults) and  phenotypes (colour morphotypes, sex).

Results

Strikingly, few genes were found to be up-regulated for high quality pearls (n = 16)  compared to the up-regulated genes in low quality pearls (n = 246). Biomineralization  genes up-regulated in low quality pearls were specific to prismatic and prism-nacre  layers. Alternative splicing was further identified in several key biomineralization genes  based on a recent P. margaritifera draft genome.

Conclusion

This study lifts the veil on the multi-level regulation of biomineralization genes 38 associated with pearl quality determination.

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How to cite 

Le Luyer Jeremy, Auffret Pauline, Quillien Virgile, Leclerc Nicolas, Reisser Celine, Vidal-Dupiol Jeremie, Ky Chin-Long (2019). Whole transcriptome sequencing and biomineralization gene architecture associated with cultured pearl quality traits in the pearl oyster, Pinctada margaritifera. Bmc Genomics, 20, -. Publisher's official version : https://doi.org/10.1186/s12864-019-5443-5 , Open Access version : https://archimer.ifremer.fr/doc/00475/58695/