Domestication and Temperature Modulate Gene Expression Signatures and Growth in the Australasian Snapper Chrysophrys auratus
|Author(s)||Wellenreuther Maren1, 2, Le Luyer Jeremy3, 4, Cook Denham1, Ritchie Peter A.5, Bernatchez Louis3|
|Affiliation(s)||1 : New Zealand Inst Plant & Food Res Ltd, Nelson, New Zealand.
2 : Univ Auckland, Sch Biol Sci, Auckland, New Zealand.
3 : Univ Laval, IBIS, Pavillon Charles Eugene Marchand, Quebec City, PQ, Canada.
4 : IFREMER, Ctr Ifremer Pacifique, Ecosyst Insulaires Oceaniens, UMR 241, BP 49, F-98725 Tahiti, France.
5 : Victoria Univ Wellington, Sch Biol Sci, Wellington, New Zealand.
|Source||G3-genes Genomes Genetics (2160-1836) (Genetics Society America), 2019-01 , Vol. 9 , N. 1 , P. 105-116|
|WOS© Times Cited||11|
|Keyword(s)||Domestication, Temperature, Transcriptomics, Growth, Sparidae|
Identifying genes and pathways involved in domestication is critical to understand how species change in response to human-induced selection pressures, such as increased temperatures. Given the profound influence of temperature on fish metabolism and organismal performance, a comparison of how temperature affects wild and domestic strains of snapper is an important question to address. We experimentally manipulated temperature conditions for F1-hatchery and wild Australasian snapper (Chrysophrys auratus) for 18 days to mimic seasonal extremes and measured differences in growth, white muscle RNA transcription and hematological parameters. Over 2.2 Gb paired-end reads were assembled de novo for a total set of 33,017 transcripts (N50 = 2,804). We found pronounced growth and gene expression differences between wild and domesticated individuals related to global developmental and immune pathways. Temperature-modulated growth responses were linked to major pathways affecting metabolism, cell regulation and signaling. This study is the first step toward gaining an understanding of the changes occurring in the early stages of domestication, and the mechanisms underlying thermal adaptation and associated growth in poikilothermic vertebrates. Our study further provides the first transcriptome resources for studying biological questions in this non-model fish species.