Discovery of a New Clade Nested Within the Genus Alexandrium (Dinophyceae): Morpho-molecular Characterization of Centrodinium punctatum (Cleve) F.J.R. Taylor

Type Article
Date 2019-04
Language English
Author(s) Li Zhun1, Mertens KennethORCID2, Nézan Elisabeth2, Chomérat Nicolas2, Bilien GwenaelORCID2, Iwataki Mitsunori3, Shin Hyeon Ho1
Affiliation(s) 1 : Library of Marine Samples, Korea Institute of Ocean Science and Technology, Geoje, Republic of Korea
2 : Ifremer, LER BO, Station de Biologie Marine,Place de la Croix, BP40537, F-29185 Concarneau Cedex, France
3 : Asian Natural Environmental Science Center, The University of Tokyo, Bunkyo, Tokyo, Japan
Source Protist (1434-4610) (Elsevier BV), 2019-04 , Vol. 170 , N. 2 , P. 168-186
DOI 10.1016/j.protis.2019.02.003
WOS© Times Cited 2
Keyword(s) Fusiform dinoflagellate, Centrodinium, Alexandrium, morphology, phylogeny
Abstract

Investigation of phytoplankton from East China Sea of the Pacific Ocean, offshore Réunion Island of the Indian Ocean, and the French Atlantic coast revealed a species of poorly known armored fusiform dinoflagellate. To clarify this species, morphology and phylogeny based on mitochondrial and nuclear protein gene sequence (Cox1, Cob and Hsp90) concatenated with the SSU, ITS region and LSU rDNA sequences were analysed. Epifluorescence and scanning electron microscopy observations revealed that the nucleus of the specimen was elongated, sausage-shaped and located equatorially on the left lateral side of the cell, and that the plate formula is Po, 3′, 1a, 6″, 6C, 8S, 5‴, 1p, 2′‴. These morphological features indicate that the species can be assigned to Centrodinium punctatum. Interestingly, the phylogenetic analyses placed this species within the Alexandrium clade, with Alexandrium affine being its closest relative. This indicates that genus Alexandrium is not monophyletic. The most similar morphological traits between C. punctatum and Alexandrium species were the shape of apical pore plate and the arrangement of the sulcal plates. However, since there are significant morphological differences between C. punctatum and Alexandrium species, further studies are needed to clarify the relation between the morphology and molecular phylogeny of other Centrodinium-related fusiform species.

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Li Zhun, Mertens Kenneth, Nézan Elisabeth, Chomérat Nicolas, Bilien Gwenael, Iwataki Mitsunori, Shin Hyeon Ho (2019). Discovery of a New Clade Nested Within the Genus Alexandrium (Dinophyceae): Morpho-molecular Characterization of Centrodinium punctatum (Cleve) F.J.R. Taylor. Protist, 170(2), 168-186. Publisher's official version : https://doi.org/10.1016/j.protis.2019.02.003 , Open Access version : https://archimer.ifremer.fr/doc/00483/59496/