Optimization of diatom Hasleao strearia cultivation in different mediums and nutrients

Type Article
Date 2019
Language English
Author(s) Arsad S1, Stavrakakis ChristopheORCID2, Turpin Vincent3, Rossa P3, Risjani Y1, Sari L A5, Prasetiya F S4, Mouget J-L6
Affiliation(s) 1 : Universitas Brawijaya, Jl. Veteran Malang 65145, Indonesia
2 : IFREMER, Station Bouin Polder des Champs, 85230 Bouin, France
3 : Université de Nantes, 1 Quai de Tourville, 44035 Nantes, France
4 : Universitas Padjajaran, Jl. Raya Bandung Sumedang Jatinangor Bandung 45363, Indonesia
5 : Universitas Airlangga, Kampus C Mulyorejo Surabaya 60115, Indonesia
6 : Le Mans Université, Avenue Olivier Messiaen 72085 Le Mans, France
Meeting 1st International Conference On Fisheries And Marine Science (InCoFiMS) : “Fisheries and Marine in Supporting Sustainable Development Goals (SDG’s) achievement”. October 2018
Source IOP Conference Series: Earth and Environmental Science (1755-1307) (IOP Publishing), 2019 , Vol. 236 , N. 1 , P. 012044 (11p.)
DOI 10.1088/1755-1315/236/1/012044

Hasleaostrearia is a marine diatom that can feed oysters. This study aimed to cultivate H.ostrearia in the following media: seawater (SW), seawater filtered by activated carbon (SW+CA), and drilling water (DW). The three-water media were supplemented with two types of nutrients, Walne-Conway+silica (CS) and ES-1/3 Provasoli (P), leading to a six experiment series. The cultures were grown in 200 mL and 400 mL respectively. The dilution cycle was every 3 - 4 day for 200 mL volume and every 4 - 5 days for the 400 mL volume for 20 - 40 days or until the cultures showed a decrease in growth. The microalgae exhibited a better growth in SW and SW+CA CS, whereas DW showed a diminishing number of cells in both the CS and P medium. The highest value of Emn in the 200 mL volume presented in the SW CS medium (1.94 mg L−1) and in the SW+CA CS medium (8.16 mg L−1), while the lowest value of Emn was found in the SW+CA CS medium (0.42 mg L−1). Imn was apparent in the SW P medium (0.56 mg L−1). In conclusion, both SW CS and SW+CA CS showed a better performance in growth. The seawater was more stable during the experiment in both the 200 mL and 400 mL volumes.

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