Nitrogen and phosphorus limitations induce carbon partitioning and membrane lipid remodelling in the marine diatom Phaeodactylum tricornutum

Type Article
Date 2019-07
Language English
Author(s) Huang Bing1, Marchand Justine1, Blanckaert Vincent2, Lukomska Ewa3, Ulmann Lionel2, Wielgosz-Collin Gaëtane4, Rabesaotra Vony4, Moreau Brigitte1, Bougaran GaelORCID3, Mimouni Virginie2, Morant-Manceau Annick1
Affiliation(s) 1 : Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), UFR Sciences et Techniques, Le Mans Université, avenue Olivier Messiaen, 72085 Le Mans cedex 09, France
2 : Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), IUT Génie Biologique, Le Mans Université, 52 rue des Docteurs Calmette et Guérin, 53020 Laval cedex 9, France
3 : Laboratoire Physiologie et Biotechnologie des Algues, IFREMER, rue de l’lle d’Yeu, BP 21105, 44311 Nantes Cedex 03, France
4 : Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), UFR Sciences pharmaceutiques et biologiques, Université de Nantes, 9 rue Bias, 44035 Nantes, France
Source European Journal Of Phycology (0967-0262) (Informa UK Limited), 2019-07 , Vol. 54 , N. 3 , P. 342-358
DOI 10.1080/09670262.2019.1567823
WOS© Times Cited 8
Keyword(s) Betaine lipid, lipid remodelling, microalga, nitrogen, phosphorus, polyunsaturated fatty acid
Abstract

Nitrogen (N) and phosphorus (P) limitations induce triacylglycerol (TAG) accumulation and membrane lipid remodelling in the marine diatom Phaeodactylum tricornutum. However, a clear understanding of the metabolic reorientation is still lacking. Carbon partitioning is of great interest because this microalga produces various highly valuable molecules such as lipids and polyunsaturated fatty acids. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of P. tricornutum throughout batch culture under N or P limitation. The integrated results show that the photosynthetic intensity was greatly reduced under N or P limitation. Under N limitation, the degradation and re-use of cellular N-containing compounds contributed to TAG accumulation, whilst P limitation favoured TAG accumulation due to the efficiency of carbon fixation, without massive degradation of essential compounds at cellular level. There was no difference in the partitioning of carbon to neutral lipids between N and P limitation. Substitution of phospholipids with betaine lipids appeared to be a P-specific acclimation strategy in P. tricornutum, which was largely regulated at the gene expression level. Betaine lipid synthesis was induced by P limitation. The lipid remodelling began once the medium became deficient in P. While the phospholipid biosynthesis pathway was not completely inhibited, a shift of lipid classes occurred immediately after their synthesis via phospholipid-recycling mechanisms.

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Huang Bing, Marchand Justine, Blanckaert Vincent, Lukomska Ewa, Ulmann Lionel, Wielgosz-Collin Gaëtane, Rabesaotra Vony, Moreau Brigitte, Bougaran Gael, Mimouni Virginie, Morant-Manceau Annick (2019). Nitrogen and phosphorus limitations induce carbon partitioning and membrane lipid remodelling in the marine diatom Phaeodactylum tricornutum. European Journal Of Phycology, 54(3), 342-358. Publisher's official version : https://doi.org/10.1080/09670262.2019.1567823 , Open Access version : https://archimer.ifremer.fr/doc/00491/60249/