Betaine lipid and neutral lipid production under nitrogen or phosphorus limitation in the marine microalga Tisochrysis lutea (Haptophyta)

Type Article
Date 2019-06
Language English
Author(s) Huang Bing1, Marchand Justine1, Thiriet-Rupert Stanislas1, Carrier GregoryORCID2, Saint-Jean BrunoORCID2, Lukomska Ewa2, Moreau Brigitte1, Morant-Manceau Annick1, Bougaran GaelORCID2, Mimouni Virginie3
Affiliation(s) 1 : Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), UFR Sciences et Techniques, Le Mans Université, avenue Olivier Messiaen, 72085 Le Mans cedex 09, France
2 : Laboratoire Physiologie et Biotechnologie des Algues, IFREMER, rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France
3 : Laboratoire Mer, Molécules, Santé (IUML – FR 3473 CNRS), IUT Département Génie Biologique, Le Mans Université, 52 rue des Docteurs Calmette et Guérin, 53020 Laval cedex 9, France
Source Algal Research-biomass Biofuels And Bioproducts (2211-9264) (Elsevier BV), 2019-06 , Vol. 40 , P. 101506 (15p.)
DOI 10.1016/j.algal.2019.101506
WOS© Times Cited 35
Keyword(s) Tisochrysis lutea, Phosphorus, Nitrogen, Lipid remodeling, Betaine lipid, Polyunsaturated fatty acid

Nitrogen (N) and phosphorus (P) limitations induce neutral lipid accumulation and membrane lipid remodeling in the domesticated oleaginous microalga Tisochrysis lutea. This study compared growth, photosynthetic activity, biochemical and transcriptional responses of T. lutea throughout batch cultures under N or P limitation compared with non-limiting nutrient condition (NP). The results show that, under N limitation, the breakdown and re-use of cellular N-containing compounds contributed to carbohydrates and further triacylglycerol (TAG) accumulation, where the accumulation of docosahexaenoic acid (DHA) in neutral lipids might mainly be derived from the recycling of membrane polar lipids. Conversely, P limitation did not hinder the photosynthetic capacity; a higher efficiency of carbon fixation fueled the allocation of carbon fluxes to the reserves of carbohydrates and neutral lipids. These latter accumulated without massive degradation of essential cellular compounds. Betaine lipids constitute the major compounds of non-plastidial membranes in T. lutea. Given an extremely low constitutive phospholipid level, phospholipids might not be involved in the regulation of P storage. However, transfer of P from non-plastidial to chloroplast membranes might occur, keeping a stable lipid composition of thylakoid membranes and maintaining a relatively high photosynthetic capacity under P deprivation.

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Huang Bing, Marchand Justine, Thiriet-Rupert Stanislas, Carrier Gregory, Saint-Jean Bruno, Lukomska Ewa, Moreau Brigitte, Morant-Manceau Annick, Bougaran Gael, Mimouni Virginie (2019). Betaine lipid and neutral lipid production under nitrogen or phosphorus limitation in the marine microalga Tisochrysis lutea (Haptophyta). Algal Research-biomass Biofuels And Bioproducts, 40, 101506 (15p.). Publisher's official version : , Open Access version :