Effect of prochloraz fungicide on biotransformation enzymes and oxidative stress parameters in three-spined stickleback (Gasterosteus aculeatus L.)

Type Article
Date 2008
Language English
Author(s) Sanchez WilfriedORCID1, Piccini Benjamin1, Porcher Jean-MarcORCID1
Affiliation(s) 1 : Inst Natl Environm Ind & Risques, Unite Evaluat Risques Ecotoxicol, Verneuil En Halatte, France.
Source Journal Of Environmental Science And Health Part B-pesticides Food Contaminants And Agricultural Wastes (0360-1234) (Taylor & Francis Inc), 2008 , Vol. 43 , N. 1 , P. 65-70
DOI 10.1080/03601230701735151
WOS© Times Cited 26
Keyword(s) biomarkers, biotransformation enzymes, liver, oxidative stress, prochloraz, three-spined stickleback
Abstract

The aim of this study was to characterize biomarker responses in three-spined sticklebacks exposed to prochloraz (Pcz). For this purpose, adult sticklebacks were exposed for 2 weeks to prochloraz at 0, 10, 50, 100 and 500 mu g/L prior to one week of depuration in clean water. At days 7, 14 and 21, several hepatic biomarkers were measured including 7-ethoxyresorufin-O-deethylase (EROD), glutathione-S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), total glutathione (GSH) content and thiobarbituric acid reactive substances (TBARS). Pcz induced a transient increase of antioxidant enzymes and a depletion of glutathione content during the first 7 days of exposure. This study showed that EROD activity and antioxidants were disrupted in a transient manner. GST was rapidly induced in a dose-dependent manner and this induction was persistent and observed also after depuration. GST appeared as a valuable biomarker to assess the exposure to Pcz.

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Sanchez Wilfried, Piccini Benjamin, Porcher Jean-Marc (2008). Effect of prochloraz fungicide on biotransformation enzymes and oxidative stress parameters in three-spined stickleback (Gasterosteus aculeatus L.). Journal Of Environmental Science And Health Part B-pesticides Food Contaminants And Agricultural Wastes, 43(1), 65-70. Publisher's official version : https://doi.org/10.1080/03601230701735151 , Open Access version : https://archimer.ifremer.fr/doc/00500/61143/