Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

Type Article
Date 2019-09
Language English
Author(s) Prieto Daniel1, Markaide Pablo1, Urrutxurtu Iñaki1, Navarro Enrique1, Artigaud Sebastien2, Fleury ElodieORCID3, Ibarrola Irrintzi1, Urrutia Miren Bego1
Affiliation(s) 1 : GIU 17/061, GI 544, Departamento de Genética, Antropología Física y Fisiología Animal, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, UPV/EHU, Apartado 644, 48080 Bilbao, Spain
2 : LEMAR UMR 6539 CNRS/UBO/IRD/Ifremer, IUEM, rue Dumont d'Urville, 29280 Plouzané, France
3 : LEMAR, UMR 6539 UBO-CNRS-Ifremer-IRD, Technopole Brest Iroise 29280 Plouzané, France
Source Aquaculture (0044-8486) (Elsevier BV), 2019-09 , Vol. 511 , P. 734242 (10p.)
DOI 10.1016/j.aquaculture.2019.734242
WOS© Times Cited 11
Keyword(s) Fast-growing, Mussel, Gill, Transcriptome

The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences.

To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups were analysed. Gene expression analysis revealed i) a low number (12) of genes differentially expressed in association with diet and ii) 117 genes differentially expressed by the fast- and slow-growing mussels. According to Biological Process GO term analysis transcriptomic differences between the F and S mussels were mainly based on the upregulation of: response to the stimulus, growth and cell activity. Regarding the KEGG terms, carbohydrate metabolism and the Krebs cycle were upregulated in F mussels, whereas biosynthetic processes were upregulated in S mussels. In accordance with their larger gill surface area and higher rates of feeding and growth, the F individuals overexpressed genes in their gill tissues, and these were involved in i) growth (insulin-like growth factors and myostatin); ii) maintenance of the structure and functioning of extracellular matrix (collagen, laminin, fibulin and decorin); iii) filtration and ciliary activity (mucin, fibrocystin, dynein and tilB homolog protein genes); iv) aerobic metabolism (citrate synthase and carbonic anhydrase); and v) the immune-system, probably in association with haemocytes. In contrast, S individuals overexpressed a different series of genes pertaining to immune system (leucine-rich repeat protein and galectin), along with genes involved in the response to cellular stress (Heat shock protein (HSP24) and metalloendopeptidase) as well as anaerobic metabolism (C4-dicarboxylate transporter). These results might suggest that S individuals would have a greater prevalence of pathogens/diseases or a higher susceptibility to the pathogens.

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