Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas

Type Article
Date 2019
Language English
Author(s) Petton BrunoORCID1, de Lorgeril JulienORCID2, Mitta GuillaumeORCID4, Daigle Gaétan3, Pernet FabriceORCID1, Alunno Bruscia MarianneORCID1
Affiliation(s) 1 : Ifremer, LEMAR UMR 6539 (Université de Bretagne Occidentale, CNRS, IRD, Ifremer), 11 presqu’île du Vivier, 29840 Argenton-en-Landunvez, France
2 : IHPE Interaction Host Pathogen Environment, UMR 5244 (Ifremer, Université de Perpignan Via Domitia, CNRS, Université de Montpellier), CC 80, 34095 Montpellier, France
3 : service de Consultation Statistique (SCS), Département de mathématiques et de statistique, Pavillon Adrien-Pouliot, Université Laval, 1065 av. de la Médecine, Québec City, Québec G1V 0A6, Canada
4 : IHPE Interaction Host Pathogen Environment, UMR 5244 (Ifremer, Université de Perpignan Via Domitia, CNRS, Université de Montpellier), CC 80, 34095 Montpellier, France
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-Research), 2019 , Vol. 135 , N. 2 , P. 97-106
DOI 10.3354/dao03384
WOS© Times Cited 5
Keyword(s) Aquaculture, Bivalve, Epidemiology, Health, Polymicrobial disease, OSHV-1, Pacific oyster mortality syndrome, POMS
Abstract

The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific pathogen-free oysters (‘recipients’) in cohabitation in the laboratory with diseased oysters that were naturally infected in the field (‘donors’). With this method, we evaluated the temporal dynamics of pathogen release in seawater and the cohabitation time necessary for disease transmission and expression. We showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation with donors was enough time to transmit pathogens to recipients and to induce mortality later, reflecting the highly contagious nature of the disease. Finally, mortality of recipients was associated with increasing cohabitation time with donors until reaching a plateau at 20%. This reflects the cumulative effect of exposure to pathogens. The optimal cohabitation time was 5−6 d, the mortality of recipients occurring 1−2 d earlier.

Full Text
File Pages Size Access
Author's final draft 28 1 MB Open access
Supplementary material 3 102 KB Open access
10 766 KB Embargo until 2024-07-25
Top of the page

How to cite 

Petton Bruno, de Lorgeril Julien, Mitta Guillaume, Daigle Gaétan, Pernet Fabrice, Alunno Bruscia Marianne (2019). Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas. Diseases Of Aquatic Organisms, 135(2), 97-106. Publisher's official version : https://doi.org/10.3354/dao03384 , Open Access version : https://archimer.ifremer.fr/doc/00507/61870/