Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale

Type Article
Date 2019-12
Language English
Author(s) Borcier Elodie1, Artigaud Sebastien1, Gaillard Jean-Charles2, Armengaud Jean2, Charrier Gregory1, Couteau Jérôme3, Receveur Justine4, Ouddane Baghdad5, Diop Mamadou6, Amara Rachid6, Laroche Jean1, Pichereau Vianney1
Affiliation(s) 1 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzane, France
2 : Laboratoire Innovations Technologiques pour la Détection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, F-30207 Bagnols-sur-Cèze, France
3 : TOXEM - Le Havre, France
4 : CEDRE – Brest, France
5 : LASIR UMR 8516 – Lille, France
6 : LOG UMR 8187 – Wimereux, France
Source Science Of The Total Environment (0048-9697) (Elsevier BV), 2019-12 , Vol. 695 , P. 133760 (13p.)
DOI 10.1016/j.scitotenv.2019.133760
WOS© Times Cited 4
Keyword(s) Estuary water quality, Ecotoxicology, Caging, Suess, Fish physiology, Shotgun proteomics
Abstract

Estuaries are important areas highly vulnerable to anthropogenic pollutions. Therefore, the assessment of estuarine water quality is a major ecological issue. In this study, we sampled juveniles of the European flounder in the “pristine” Canche estuary, and caged them in Canche and in two polluted sites of the Seine estuary, Rouen and Fosse Nord. After one month, the metal and organic pollutants in these sites were assessed, and we evaluated several phenotypic indicators (condition index, RNA/DNA ratios and genotoxicity), and extracted the proteins in fish livers for analysis using a shotgun proteomics approach.

The results showed strong modifications in the fish caged in both sites of the Seine estuary, as compared to those caged in Canche. In particular, many proteins involved in phase I and phase II detoxification reactions were accumulated in the liver of fish caged in the site showing the highest pollution, Rouen. In addition, we observed a general disruption of metabolism, in particular an increase in lipid synthesis and carbohydrate degradation in Rouen, and a decrease in the abundance of proteins associated to translational activity in Fosse Nord. At both sites, several stress proteins were decreased.

The proteomic impact of the encagement by itself was also evaluated, by comparing the liver proteome of fish caged in Canche to that of fish stayed in natura during the same time. The results showed proteomic signatures of exposure to stressful conditions (particularly heat stress), most probably related to the micro-habitat in which the cages were placed. In conclusion, the caging technique is of great interest for ecotoxicological assessment of estuarine waters, but should consider that the results are representative of the micro-habitat around the cages, which does not necessarily represent the overall heterogeneity of the estuarine environment.

Full Text
File Pages Size Access
Author's final draft 50 1 MB Open access
13 1 MB Access on demand
33 MB Access on demand
Top of the page

How to cite 

Borcier Elodie, Artigaud Sebastien, Gaillard Jean-Charles, Armengaud Jean, Charrier Gregory, Couteau Jérôme, Receveur Justine, Ouddane Baghdad, Diop Mamadou, Amara Rachid, Laroche Jean, Pichereau Vianney (2019). Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale. Science Of The Total Environment, 695, 133760 (13p.). Publisher's official version : https://doi.org/10.1016/j.scitotenv.2019.133760 , Open Access version : https://archimer.ifremer.fr/doc/00509/62067/