Resistance of the oyster pathogen Vibrio tasmaniensis LGP32 against grazing by Vannella sp . marine amoeba involves Vsm and CopA virulence factors

Type Article
Date 2020-10
Language English
Author(s) Robino Etienne1, Poirier Aurore C.5, Amraoui Hajar5, Le Bissonnais Sandra5, Perret Angelique1, Lopez‐joven Carmen, Auguet Jean-Christophe2, Rubio Tristan P.5, Cazevieille Chantal3, Rolland Jean-Luc1, Héchard Yann4, Destoumieux‐garzón Delphine5, Charrière Guillaume5
Affiliation(s) 1 : IHPE UMR 5244, CNRS, Ifremer, University of Montpellier, University of Perpignan Via Domitia Montpellier, France
2 : MARBEC, University of Montpellier, CNRS, Ifremer, IRD Montpellier, France
3 : INM, University of Montpellier Montpellier, France
4 : EBI UMR CNRS 7267, University of Poitiers Poitiers, France
5 : IHPE UMR 5244, CNRS, Ifremer, University of Montpellier, University of Perpignan Via Domitia Montpellier, France
Source Environmental Microbiology (1462-2912) (Wiley), 2020-10 , Vol. 22 , N. 10 , P. 4183-4197
DOI 10.1111/1462-2920.14770
WOS© Times Cited 4
Note Special Issue on Vibrios – from genes to ecosystems
Abstract

ibrios are ubiquitous in marine environments and opportunistically colonize a broad range of hosts. Strains of Vibrio tasmaniensis present in oyster farms can thrive in oysters during juvenile mortality events and behave as facultative intracellular pathogen of oyster hemocytes. Herein, we wondered whether V. tasmaniensis LGP32 resistance to phagocytosis is specific to oyster immune cells or contributes to resistance to other phagocytes, like marine amoebae. To address this question, we developed an integrative study, from the first description of amoeba diversity in oyster‐farms to the characterization of LGP32 interactions with amoebae. An isolate of the Vannella genus, Vannella sp. AP1411, which was collected from oyster farms, is ubiquitous, and belongs to one clade of Vannella that could be found associated with Vibrionaceae. LGP32 was shown to be resistant to grazing by Vannella sp. AP1411 and this phenotype depends on some previously identified virulence factors: secreted metalloprotease Vsm and copper efflux p‐ATPase CopA, which act at different steps during amoeba‐vibrio interactions, whereas some other virulence factors were not involved. Altogether, our work indicate that some virulence factors can by involved in multi‐host interactions of V. tasmaniensis ranging from protozoans to metazoans, potentially favoring their opportunistic behavior.

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Robino Etienne, Poirier Aurore C., Amraoui Hajar, Le Bissonnais Sandra, Perret Angelique, Lopez‐joven Carmen, Auguet Jean-Christophe, Rubio Tristan P., Cazevieille Chantal, Rolland Jean-Luc, Héchard Yann, Destoumieux‐garzón Delphine, Charrière Guillaume (2020). Resistance of the oyster pathogen Vibrio tasmaniensis LGP32 against grazing by Vannella sp . marine amoeba involves Vsm and CopA virulence factors. Environmental Microbiology, 22(10), 4183-4197. Publisher's official version : https://doi.org/10.1111/1462-2920.14770 , Open Access version : https://archimer.ifremer.fr/doc/00509/62109/