Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios

Type Article
Date 2019-09
Language English
Author(s) Wegner K. Mathias1, Piel Damien2, 3, Bruto Maxime3, John Uwe4, 5, Mao Zhijuan6, Alunno-Bruscia MarianneORCID2, 7, Petton BrunoORCID2, 7, Le Roux Frederique2, 3
Affiliation(s) 1 : Coastal Ecology, Waddensea Station Sylt, Alfred Wegener Institut - Helmholtz-Zentrum für Polar- und Meeresforschung, List, Germany
2 : Unité Physiologie Fonctionnelle des Organismes Marins, Ifremer, Plouzané, France
3 : Integrative Biology of Marine Models, UPMC Paris 06, CNRS, UMR 8227, Sorbonne Universités, Station Biologique de Roscoff, Roscoff, France
4 : Ecolgical Chemistry, Alfred Wegener Institut - Helmholtz-Zentrum für Polar- und Meeresforschung, Bremerhaven, Germany
5 : Helmholtz Institute for Functional Marine Biodiversity (HIFMB), Oldenburg, Germany
6 : Biological and Environmental College, Zhejiang Wanli University, Ningbo, China
7 : LEMAR UMR 6539, Ifremer, Argenton-en-Landunvez, France
Source Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2019-09 , Vol. 10 , N. 2067 , P. 13p.
DOI 10.3389/fmicb.2019.02067
WOS© Times Cited 5
Keyword(s) virulence, host-pathogen interaction, local adaptation, biological invasion, emerging disease, Wadden Sea

Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between different host populations and their locally encountered Vibrio communities. Here, we explore the molecular targets of such co-evolutionary interactions by analyzing the genomes of nine Vibrio strains from the Splendidus-clade showing opposite virulence patterns towards two populations of Pacific oysters introduced into European Wadden Sea. By contrasting Vibrio phylogeny to their host specific virulence patterns, we could identify two core genome genes (OG1907 and OG 3159) that determine the genotype by genotype (G × G) interactions between oyster larvae and their sympatric Vibrio communities. Both genes show positive selection between locations targeting only few amino acid positions. Deletion of each gene led to a loss of the host specific virulence patterns while complementation with OG3159 alleles from both locations could recreate the wild type phenotypes matching the origin of the allele. This indicates that both genes can act as a genetic switch for Vibrio-oyster coevolution demonstrating that local adaptation in distinct Vibrio lineages can rely on only few genes independent of larger pathogenicity islands or plasmids.

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Table 1 Primers used in the present study. 37 KB Open access
Table 2 Strains and plasmids used or constructed in the present study. 19 KB Open access
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Wegner K. Mathias, Piel Damien, Bruto Maxime, John Uwe, Mao Zhijuan, Alunno-Bruscia Marianne, Petton Bruno, Le Roux Frederique (2019). Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios. Frontiers In Microbiology, 10(2067), 13p. Publisher's official version : , Open Access version :