Towards the optimization of botanical insecticides research: Aedes aegypti larvicidal natural products in French Guiana

Type Article
Date 2020-01
Language English
Author(s) Falkowski Michaël1, Jahn-Oyac Arnaud1, Odonne Guillaume2, Flora Claudiane1, Estevez Yannick1, Touré Seindé1, 3, Boulogne Isabelle1, 4, 5, Robinson Jean-Charles6, Béreau Didier6, Petit Philippe4, Azam Didier7, Coke Maïra7, Issaly Jean8, Gaborit Pascal8, Stien Didier9, Eparvier Véronique3, Dusfour Isabelle8, 10, Houël Emeline1
Affiliation(s) 1 : CNRS, UMR EcoFoG, AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, 97300 Cayenne, France
2 : Laboratoire Ecologie, Evolution, Interactions des Systèmes Amazoniens (LEEISA), CNRS, Université de Guyane, IFREMER, 97300 Cayenne, France
3 : CNRS, Institut de Chimie des Substances Naturelles, UPR2301, Université Paris Saclay, 91198 Gif-sur-Yvette, France
4 : Université des Antilles, Campus de Fouillole, 97157 Pointe-à-Pitre Cedex, Guadeloupe, France
5 : Université de ROUEN, UFR des Sciences et Techniques, Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie Végétal » FED 4277, 76821, Mont-Saint-Aignan, France.
6 : Université de Guyane, Laboratoire COVAPAM, UMR QualiSud, Campus universitaire de Troubiran, BP 792, 97337 Cayenne cedex, France
7 : Unité Expérimentale d'Ecologie et d'Ecotoxicologie Aquatique, INRA-U3E, 35042 Rennes, France
8 : Institut Pasteur de la Guyane, Vectopôle Amazonien Emile Abonnenc, Unité Contrôle et Adaptation des vecteurs, 23 avenue Pasteur, BP6010, 97306 Cayenne cedex, France
9 : Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologie Microbienne, LBBM, Observatoire Océanologique, 66650 Banyuls-sur-mer, France
10 : INRS-Institut Armand Frappier, Groupe recherche en écologie microbienne, 531 boulevard des prairies, H7V 1B7, Laval, QC, Canada
Source Acta Tropica (0001-706X) (Elsevier BV), 2020-01 , Vol. 201 , P. 105179 (12p.)
DOI 10.1016/j.actatropica.2019.105179
WOS© Times Cited 3
Keyword(s) Mosquito larvicides, Culicidae, Amazonian chemodiversity, Screening optimization, Quasi-Poisson generalized linear model, Chemical defense
Abstract

Natural products have proven to be an immeasurable source of bioactive compounds. The exceptional biodiversity encountered in Amazonia, alongside a rich entomofauna and frequent interactions with various herbivores is the crucible of a promising chemodiversity. This prompted us to search for novel botanical insecticides in French Guiana. As this French overseas department faces severe issues linked to insects, notably the strong incidence of vector-borne infectious diseases, we decided to focus our research on products able to control the mosquito Aedes aegypti. We tested 452 extracts obtained from 85 species originating from 36 botanical families and collected in contrasted environments against an Aedes aegypti laboratory strain susceptible to all insecticides, and a natural population resistant to both pyrethroid and organophosphate insecticides collected in Cayenne for the most active of them. 8 species (Maytenus oblongata Reissek, Celastraceae; Costus erythrothyrsus Loes., Costaceae; Humiria balsamifera Aubl., Humiriaceae; Sextonia rubra (Mez) van der Werff, Lauraceae; Piper hispidum Sw., Piperaceae; Laetia procera (Poepp.) Eichl., Salicaceae; Matayba arborescens (Aubl.) Radlk., Sapindaceae; and Cupania scrobitulata Rich., Sapindaceae) led to extracts exhibiting more than 50% larval mortality after 48h of exposition at 100 µg/mL against the natural population and were considered active. Selectivity and phytochemistry of these extracts were therefore investigated and discussed, and some active compounds highlighted. Multivariate analysis highlighted that solvents, plant tissues, plant family and location had a significant effect on mortality while light, available resources and vegetation type did not. Through this case study we highlighted that plant defensive chemistry mechanisms are crucial while searching for novel insecticidal products.

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Falkowski Michaël, Jahn-Oyac Arnaud, Odonne Guillaume, Flora Claudiane, Estevez Yannick, Touré Seindé, Boulogne Isabelle, Robinson Jean-Charles, Béreau Didier, Petit Philippe, Azam Didier, Coke Maïra, Issaly Jean, Gaborit Pascal, Stien Didier, Eparvier Véronique, Dusfour Isabelle, Houël Emeline (2020). Towards the optimization of botanical insecticides research: Aedes aegypti larvicidal natural products in French Guiana. Acta Tropica, 201, 105179 (12p.). Publisher's official version : https://doi.org/10.1016/j.actatropica.2019.105179 , Open Access version : https://archimer.ifremer.fr/doc/00514/62544/