Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass
Type | Article | ||||||||||||||||||||
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Date | 2019-10 | ||||||||||||||||||||
Language | English | ||||||||||||||||||||
Author(s) | Masroor Waliullah1, Farcy Emilie1, Blondeau-Bidet Eva3, Venn Alexander2, Tambutté Eric2, Lorin-Nebel Catherine1 | ||||||||||||||||||||
Affiliation(s) | 1 : Univ Montpellier, CNRS, IFREMER, IRD, UM, MARBEC, France 2 : Centre Scientifique de Monaco, Marine Biology Department, 8 Quai Antoine 1er, 98000, Monaco 3 : Univ Montpellier, CNRS, IFREMER, IRD, UM, MARBEC, France |
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Source | Journal Of Thermal Biology (0306-4565) (Elsevier BV), 2019-10 , Vol. 85 , P. 102422 (9p.) | ||||||||||||||||||||
DOI | 10.1016/j.jtherbio.2019.102422 | ||||||||||||||||||||
WOS© Times Cited | 14 | ||||||||||||||||||||
Keyword(s) | Dicentrarchus labrax, Ion transporter, Gill, mRNA expression, Thermal acclimation, Salinity | ||||||||||||||||||||
Abstract | The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 °C (temperate) or 24 °C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase α1a (nka α1a) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl−-cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl--1 cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level. |
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