Intracellular Infection of Diverse Diatoms by an Evolutionary Distinct Relative of the Fungi

Type Article
Date 2019-12
Language English
Author(s) Chambouvet Aurélie1, Monier Adam2, Maguire Finlay2, 3, Itoïz Sarah1, Del Campo Javier4, Elies Philippe5, Edvardsen Bente6, Eikreim Wenche6, Richards Thomas A.2
Affiliation(s) 1 : CNRS, Univ Brest, IRD, Ifremer, LEMAR, F-29280 Plouzane, France
2 : Living Systems Institute, School of Biosciences, University of Exeter, Exeter, EX4 4QD, UK
3 : Faculty of Computer Science, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada
4 : Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, FL, 33149 USA
5 : Plateforme d’Imagerie et de Mesures en Microscopie, Université de Bretagne Occidentale, 29200, Brest, France
6 : Section for Aquatic Biology and Toxicology, Department of Biosciences, University of Oslo, PO Box 1066 Blindern, 0316 Oslo, Norway
Source Current Biology (0960-9822) (Elsevier BV), 2019-12 , Vol. 29 , N. 23 , P. 4093-4101.e4
DOI 10.1016/j.cub.2019.09.074
WOS© Times Cited 5
Abstract

The Fungi are a diverse kingdom, dominating terrestrial environments and driving important ecologies. Although fungi, and the related Opisthosporidia, interact with photosynthetic organisms on land and in freshwater as parasites, symbionts, and/or saprotrophic degraders [1, 2], such interactions in the marine environment are poorly understood [3, 4, 5, 6, 7, 8]. One newly identified uncultured marine lineage has been named novel chytrid-like-clade-1 (NCLC1) [4] or basal-clone-group-I [5, 6]. We use ribosomal RNA (rRNA) encoding gene phylogenies to demonstrate that NCLC1 is a distinct branch within the Opisthosporidia (Holomycota) [7]. Opisthosporidia are a diverse and largely uncultured group that form a sister branch to the Fungi or, alternatively, the deepest branch within the Fungi, depending on how the boundary to this kingdom is inferred [9]. Using culture-free lineage-specific rRNA-targeted fluorescent in situ hybridization (FISH) microscopy, we demonstrate that NCLC1 cells form intracellular infection of key diatom species, establishing that intracellular colonization of a eukaryotic host is a consistent lifestyle across the Opisthosporidia [8, 9, 10, 11]. NCLC1 infection-associated loss and/or envelopment of the diatom nuclei infers a necrotrophic-pathogenic interaction. Diatoms are one of the most diverse and ecologically important phytoplankton groups, acting as dominant primary producers and driving carbon fixation and storage in many aquatic environments [12, 13, 14]. Our results provide insight into the diversity of microbial eukaryotes that interact with diatoms. We suggest that such interactions can play a key role in diatom associated ecosystem functions, such as the marine carbon pump through necrotrophic-parasitism, facilitating the export of diatoms to the sediment [15, 16].

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How to cite 

Chambouvet Aurélie, Monier Adam, Maguire Finlay, Itoïz Sarah, Del Campo Javier, Elies Philippe, Edvardsen Bente, Eikreim Wenche, Richards Thomas A. (2019). Intracellular Infection of Diverse Diatoms by an Evolutionary Distinct Relative of the Fungi. Current Biology, 29(23), 4093-4101.e4. Publisher's official version : https://doi.org/10.1016/j.cub.2019.09.074 , Open Access version : https://archimer.ifremer.fr/doc/00591/70337/