Campylobacter armoricus sp. nov., a novel member of the Campylobacter lari group isolated from surface water and stools from humans with enteric infection

Type Article
Date 2019-12
Language English
Author(s) Boukerb Amine M.1, Penny Christian2, Serghine Joelle1, Walczak Cécile2, Cauchie Henry-Michel2, Miller William G.3, Losch Serge4, Ragimbeau Catherine5, Mossong Joël5, Mégraud Francis6, 7, Lehours Philippe6, 7, Bénéjat Lucie7, Gourmelon MicheleORCID1
Affiliation(s) 1 : Ifremer, RBE-SGMM-LSEM, Laboratoire Santé Environnement Microbiologie, Plouzané, France
2 : Luxembourg Institute of Science and Technology (LIST), Environmental Research and Innovation Department, Esch-sur-Alzette, Luxembourg
3 : US Department of Agriculture, Produce Safety and Microbiology Research Unit, Agricultural Research Service, Albany, CA, USA
4 : Laboratoire de Médecine Vétérinaire de l’Etat (LMVE), Veterinary Services Administration, Dudelange, Luxembourg
5 : Laboratoire National de Santé (LNS), Epidemiology and Microbial Genomics, Dudelange, Luxembourg
6 : INSERM, University of Bordeaux, UMR1053 Bordeaux Research In Translational Oncology, BaRITOn, Bordeaux, France
7 : French National Reference Centre for Campylobacter and Helicobacter, Pellegrin University Hospital, Bordeaux, France
Source International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Microbiology Society), 2019-12 , Vol. 69 , N. 12 , P. 3969-3979
DOI 10.1099/ijsem.0.003836
WOS© Times Cited 2
Keyword(s) Campylobacter, novel species, surface water, coastal catchment, human gastroenteritis, C. lari group, whole genorne sequence
Abstract

During a study on the prevalence and diversity of members of the genus Campylobacter in a shellfish-harvesting area and its catchment in Brittany, France, six urease-positive isolates of members of the genus Campylobacter were recovered from surface water samples, as well as three isolates from stools of humans displaying enteric infection in the same period. These strains were initially identified as members of the Campylobacter lari group by MALDI-TOF mass spectrometry and placed into a distinct group in the genus Campylobacter, following atpA gene sequence analysis based on whole-genome sequencing data. This taxonomic position was confirmed by phylogenetic analysis of the 16S rRNA, rpoB and hsp60 (groEL) loci, and an analysis of the core genome that provided an improved phylogenetic resolution. The average nucleotide identity between the representative strain CA656T (CCUG 73571T=CIP 111675T) and the type strain of the most closely related species Campylobacter ornithocola WBE38T was 88.5 %. The strains were found to be microaerobic and anaerobic, motile, non-spore-forming, Gram-stain-negative, spiral-shaped bacteria that exhibit catalase, oxidase and urease activities but not nitrate reduction. This study demonstrates clearly that the nine isolates represent a novel species within the C. lari group, for which the name Campylobacter armoricus is proposed. Here, we present phenotypic and morphological features of the nine strains and the description of their genome sequences. The proposed type strain CA656T has a 1.589 Mbp chromosome with a DNA G+C content of 28.5 mol% and encodes 1588 predicted coding sequences, 38 tRNAs, and 3 rRNA operons.

 

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Boukerb Amine M., Penny Christian, Serghine Joelle, Walczak Cécile, Cauchie Henry-Michel, Miller William G., Losch Serge, Ragimbeau Catherine, Mossong Joël, Mégraud Francis, Lehours Philippe, Bénéjat Lucie, Gourmelon Michele (2019). Campylobacter armoricus sp. nov., a novel member of the Campylobacter lari group isolated from surface water and stools from humans with enteric infection. International Journal Of Systematic And Evolutionary Microbiology, 69(12), 3969-3979. Publisher's official version : https://doi.org/10.1099/ijsem.0.003836 , Open Access version : https://archimer.ifremer.fr/doc/00591/70346/