Low-diversity bacterial microbiota in Southern Ocean representatives of lanternfish genera Electrona, Protomyctophum and Gymnoscopelus (family Myctophidae)

Type Article
Date 2019-12
Language English
Author(s) Gallet Alison1, Koubbi Philippe2, 3, Léger Nelly4, Scheifler Mathilde5, Ruiz-Rodriguez Magdalena6, Suzuki Marcelino T.7, Desdevises Yves8, Duperron Sebastien9
Affiliation(s) 1 : Muséum National d’Histoire Naturelle, CNRS, Molécules de Communication et Adaptation des Micro-organismes, MCAM, Muséum national d’Histoire naturelle, Paris, France
2 : IFREMER, Channel and North Sea Fisheries Research Unit, Boulogne-sur-Mer, France
3 : UFR 918 « Terre, Environnement, Biodiversité », Sorbonne Université, place Jussieu, Paris, France
4 : Sorbonne Université, Biologie des Organismes et Ecosystèmes Aquatiques BOREA, Paris, France
5 : Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France
6 : Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France
7 : Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes, LBBM Observatoire Océanologique, Banyuls/Mer, France
8 : Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France
9 : Muséum National d’Histoire Naturelle, CNRS, Molécules de Communication et Adaptation des Micro-organismes, MCAM, Muséum national d’Histoire naturelle, Paris, France, Institut Universitaire de France, Paris, France
Source Plos One (1932-6203) (Public Library of Science (PLoS)), 2019-12 , Vol. 14 , N. 12 , P. e0226159 (17p.)
DOI 10.1371/journal.pone.0226159
WOS© Times Cited 2
Abstract

Myctophids are among the most abundant mesopelagic teleost fishes worldwide. They are dominant in the Southern Ocean, an extreme environment where they are important both as consumers of zooplankton as well as food items for larger predators. Various studies have investigated myctophids diet, but no data is yet available regarding their associated microbiota, despite that the significance of bacterial communities to fish health and adaptation is increasingly acknowledged. In order to document microbiota in key fish groups from the Southern Ocean, the bacterial communities associated with the gut, fin, gills and light organs of members of six species within the three myctophid genera Electrona, Protomyctophum and Gymnoscopelus were characterized using a 16S rRNA-based metabarcoding approach. Gut communities display limited diversity of mostly fish-specific lineages likely involved in food processing. Fin and skin communities display diversity levels and compositions resembling more those found in surrounding seawater. Community compositions are similar between genera Electrona and Protomyctophum, that differ from those found in Gymnoscopelus and in water. Low abundances of potentially light-emitting bacteria in light organs support the hypothesis of host production of light. This first description of myctophid-associated microbiota, and among the first on fish from the Southern Ocean, emphasizes the need to extend microbiome research beyond economically-important species, and start addressing ecologically-relevant species.

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S1 Fig. Rarefaction curves by sample type, indicating that samples of animal origin (gill, fin, intestine and luminous organ) reach saturation at the selected 1,100 sequencing depth. 56 KB Open access
S1 Table. Results from Kruskal-Wallis tests done on Faith Phylogenetic Diversity (sheet 1) and PERMANOVA analyses (sheet 2) conducted to test the effect of genus, tissue and date, and ... 9 KB Open access
S2 Table. Summary of identified ASVs and their percentage occurrence in each individual sample; maximum observed percentage, taxonomic affiliation and sequence are provided. 609 KB Open access
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How to cite 

Gallet Alison, Koubbi Philippe, Léger Nelly, Scheifler Mathilde, Ruiz-Rodriguez Magdalena, Suzuki Marcelino T., Desdevises Yves, Duperron Sebastien (2019). Low-diversity bacterial microbiota in Southern Ocean representatives of lanternfish genera Electrona, Protomyctophum and Gymnoscopelus (family Myctophidae). Plos One, 14(12), e0226159 (17p.). Publisher's official version : https://doi.org/10.1371/journal.pone.0226159 , Open Access version : https://archimer.ifremer.fr/doc/00598/70973/