First evaluation of resistance to both a California OsHV-1 variant and a French OsHV-1 microvariant in Pacific oysters

Type Article
Date 2019-12
Language English
Author(s) Divilov Konstantin1, Schoolfield Blaine1, Morga BenjaminORCID2, Dégremont LionelORCID2, Burge Colleen A.3, Mancilla Cortez Daniel4, Friedman Carolyn S.5, Fleener Gary B.4, Dumbauld Brett R.6, Langdon Chris1
Affiliation(s) 1 : Department of Fisheries and Wildlife, Coastal Oregon Marine Experiment Station, Oregon State University, Hatfield Marine Science Center, Newport, Oregon, USA
2 : Laboratoire de Génétique et Pathologie des Mollusques Marins, Ifremer, La Tremblade, France
3 : Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA
4 : Hog Island Oyster Co., Marshall, California, USA
5 : School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington, USA
6 : United States Department of Agriculture-Agricultural Research Service, Hatfield Marine Science Center, Newport, Oregon, USA
Source Bmc Genetics (1471-2156) (Springer Science and Business Media LLC), 2019-12 , Vol. 20 , N. 1 , P. 96 (9p.)
DOI 10.1186/s12863-019-0791-3
WOS© Times Cited 15
Keyword(s) Ostreid herpesvirus 1, Crassostrea gigas, Heritability, Breeding
Abstract

Background

Variants of the Ostreid herpesvirus 1 (OsHV-1) cause high losses of Pacific oysters globally, including in Tomales Bay, California, USA. A suite of new variants, the OsHV-1 microvariants (μvars), cause very high mortalities of Pacific oysters in major oyster-growing regions outside of the United States. There are currently no known Pacific oysters in the United States that are resistant to OsHV-1 as resistance has yet to be evaluated in these oysters. As part of an effort to begin genetic selection for resistance to OsHV-1, 71 families from the Molluscan Broodstock Program, a US West Coast Pacific oyster breeding program, were screened for survival after exposure to OsHV-1 in Tomales Bay. They were also tested in a quarantine laboratory in France where they were exposed to a French OsHV-1 microvariant using a plate assay, with survival recorded from three to seven days post-infection.

Results

Significant heritability for survival were found for all time points in the plate assay and in the survival phenotype from a single mortality count in Tomales Bay. Genetic correlations between survival against the French OsHV-1 μvar in the plate assay and the Tomales Bay variant in the field trait were weak or non-significant.

Conclusions

Future breeding efforts will seek to validate the potential of genetic improvement for survival to OsHV-1 through selection using the Molluscan Broodstock Program oysters. The lack of a strong correlation in survival between OsHV-1 variants under this study’s exposure conditions may require independent selection pressure for survival to each variant in order to make simultaneous genetic gains in resistance.

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How to cite 

Divilov Konstantin, Schoolfield Blaine, Morga Benjamin, Dégremont Lionel, Burge Colleen A., Mancilla Cortez Daniel, Friedman Carolyn S., Fleener Gary B., Dumbauld Brett R., Langdon Chris (2019). First evaluation of resistance to both a California OsHV-1 variant and a French OsHV-1 microvariant in Pacific oysters. Bmc Genetics, 20(1), 96 (9p.). Publisher's official version : https://doi.org/10.1186/s12863-019-0791-3 , Open Access version : https://archimer.ifremer.fr/doc/00598/70974/