Potential of fascaplysin and palauolide from Fascaplysinopsis cf reticulata to reduce the risk of bacterial infection in fish farming
Type | Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Date | 2019-12 | ||||||||
Language | English | ||||||||
Author(s) | Mai Tepoerau1, Toullec Jordan11, Van Wynsberge Simon1, 3, 4, Besson Marc5, 6, Soulet Stephanie12, Petek Sylvain13, Aliotti Emmanuelle13, Ekins Merrick7, 8, Hall Kathryn7, Erpenbeck Dirk9, Lecchini David5, Beniddir Mehdi A.10, Saulnier Denis1, Debitus Cecile13 | ||||||||
Affiliation(s) | 1 : EIO, ILM, IFREMER, IRD, UPF, BP 6570, 98702 FAA’A, Tahiti, French Polynesia 2 : IRD, Univ. Brest, CNRS, IFREMER, LEMAR, F-29280 Plouzane, France 3 : Institut de Recherche pour le Développement, UMR 9220 ENTROPIE (Institut de Recherche Pour le Développement, Université de la Réunion, Centre National de la Recherche Scientifique), Nouméa, New Caledonia 4 : LEAD-NC, 98848 Nouméa, New Caledonia 5 : PSL Research University: EPHE-UPVD-CNRS, USR 3278 CRIOBE, BP 1013-Moorea, French Polynesia 6 : Observatoire Océanologique de Banyuls-sur-Mer, Université Pierre et Marie Curie Paris, Banyuls-sur-Mer, France 7 : Queensland Museum, PO Box 3300, South Brisbane BC, Queensland 4101, Australia 8 : School of Biological Sciences, University of Queensland, St Lucia, Queensland, 4072, Australia 9 : Department of Earth and Environmental Sciences & GeoBio-Center, Ludwig-Maximilians-Universität München, Richard-Wagner Str. 10, 80333 Munich, Germany 10 : Biocis, Equipe “Pharmacognosie-Chimie des substances naturelles”, CNRS, Université de Paris Sus, Univ. Paris-Saclay, Faculté de pharmacie, 5 rue J-B Clément, 92293 Châtenay-Malabry cedex, France 11 : EIO, ILM, IFREMER, IRD, UPF, BP 6570, 98702 FAA’A, Tahiti, French Polynesia 12 : EIO, ILM, IFREMER, IRD, UPF, BP 6570, 98702 FAA’A, Tahiti, French Polynesia 13 : EIO, ILM, IFREMER, IRD, UPF, BP 6570, 98702 FAA’A, Tahiti, French Polynesia |
||||||||
Source | Fisheries and Aquatic Sciences (2234-1757) (Springer Science and Business Media LLC), 2019-12 , Vol. 22 , N. 1 , P. 30 (11p.) | ||||||||
DOI | 10.1186/s41240-019-0145-0 | ||||||||
Keyword(s) | Porifera, Marine natural products, Quorum sensing inhibitors, Antibiotic, Fascaplysinopsis cf reticulata | ||||||||
Abstract | Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2, and CAI-1. Bioassay-guided purification of F. cf reticulata extract was conducted on two bacterial species, i.e., Tenacibaculum maritimum and V. harveyi for antibiotic and QS inhibition bioactivities. Toxicity bioassay of fractions was also evaluated on the freshwater fish Poecilia reticulata and the marine fish Acanthurus triostegus. Cyclohexanic and dichloromethane fractions of F. cf reticulata exhibited QS inhibition on V. harveyi and antibiotic bioactivities on V. harveyi and T. maritimum, respectively. Palauolide (1) and fascaplysin (2) were purified as major molecules from the cyclohexanic and dichloromethane fractions, respectively. Palauolide inhibited QS of V. harveyi through HAI-1 QS pathway at 50 μg ml–1 (26 μM), while fascaplysin affected the bacterial growth of V. harveyi (50 μg ml–1) and T. maritimum (0.25 μg). The toxicity of fascaplysin-enriched fraction (FEF) was evaluated and exhibited a toxic effect against fish at 50 μg ml–1. This study demonstrated for the first time the QSI potential of palauolide (1). Future research may assess the toxicity of both the cyclohexanic fraction of the sponge and palauolide (1) on fish, to confirm their potential as alternative to antibiotics in fish farming. |
||||||||
Full Text |
|