Physiological responses during acute stress recovery depend on stress coping style in European sea bass, Dicentrarchus labrax.

Type Article
Date 2020-03
Language English
Author(s) Ferrari Sebastien1, 2, Rey Sonia3, Høglund Erik4, Øverli Øyvind5, Chatain Beatrice1, Mackenzie Simon3, Bégout Marie-LaureORCID1
Affiliation(s) 1 : Ifremer, Fisheries Research Laboratory, L'Houmeau, 17137, France
2 : MARBEC, Ifremer, Univ. Montpellier, CNRS, IRD, Palavas-les-flots, France
3 : Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
4 : Norwegian Institute for Water Research (NIVA), Oslo, N-0349, Norway
5 : Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, N-0033, Norway
Source Physiology & Behavior (0031-9384) (Elsevier BV), 2020-03 , Vol. 216 , P. 112801 (9p.)
DOI 10.1016/j.physbeh.2020.112801
WOS© Times Cited 1
Keyword(s) Fish, Behavior, Physiology, Personality, Brain, Transcriptomic
Abstract

Individual stress coping style (reactive, intermediate and proactive) was determined in 3 groups of 120 pit tagged European seabass using the hypoxia avoidance test. The same three groups (no change in social composition) were then reared according to the standards recommended for this species. Then, 127 days later, individuals initially characterized as reactive, intermediate or proactive were submitted to an acute confinement stress for 30 min. Blood samples were taken to measure plasma cortisol levels 30 min (Stress30) or 150 min (Stress150) after the end of the confinement stress. Individuals were then sacrificed to sample the telencephalon in order to measure the main monoamines and their catabolites (at Stress30 only). Individuals from Stress150 were sampled for whole brain for a transcriptomic analysis. The main results showed that reactive individuals had a lower body mass than intermediate individuals which did not differ from proactive individuals. The physiological cortisol response did not differ between coping style at Stress30 but at Stress150 when intermediate and proactive individuals had recovered pre stress levels, reactive individuals showed a significant higher level illustrating a modulation of stress recovery by coping style. Serotonin turnover ratio was higher in proactive and reactive individuals compared to intermediate individuals and a significant positive correlation was observed with cortisol levels whatever the coping style. Further, the confinement stress led to a general increase in the serotonin turnover comparable between coping styles. Stress150 had a significant effect on target mRNA copy number (Gapdh mRNA copy number decreased while ifrd1 mRNA copy number increased) and such changes tended to depend upon coping style.

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Ferrari Sebastien, Rey Sonia, Høglund Erik, Øverli Øyvind, Chatain Beatrice, Mackenzie Simon, Bégout Marie-Laure (2020). Physiological responses during acute stress recovery depend on stress coping style in European sea bass, Dicentrarchus labrax. Physiology & Behavior, 216, 112801 (9p.). Publisher's official version : https://doi.org/10.1016/j.physbeh.2020.112801 , Open Access version : https://archimer.ifremer.fr/doc/00601/71330/