Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome
|Author(s)||De Lorgeril Julien1, Petton Bruno1, Lucasson Aude1, Perez Valérie1, Stenger Pierre-Louis1, Degremont Lionel1, Montagnani Caroline1, Escoubas Jean Michel2, Haffner Philippe1, Allienne Jean-François2, Leroy Marc2, Lagarde Franck1, Vidal Dupiol Jeremie1, Gueguen Yannick1, Mitta Guillaume3|
|Affiliation(s)||1 : Ifremer, france
2 : CNRS, france
3 : Univ perpignan, France
|Source||Bmc Genomics (1471-2164) (Research Square), 2020-01 , Vol. 21 , N. 1 , P. 63 (14p.)|
|Keyword(s)||Pacific oyster, Oyster disease, Resistance, OsHV-1, Antiviral molecular pathways, Invertebrate immunity|
Background: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. Conclusions: We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function.