Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome

Type Article
Date 2020-01
Language English
Author(s) de Lorgeril JulienORCID1, Petton BrunoORCID1, Lucasson Aude1, Perez Valérie1, Stenger Pierre-Louis1, Degremont LionelORCID1, Montagnani CarolineORCID1, Escoubas Jean Michel2, Haffner Philippe1, Allienne Jean-François2, Leroy Marc2, Lagarde FranckORCID1, Vidal Dupiol JeremieORCID1, Gueguen YannickORCID1, Mitta GuillaumeORCID3
Affiliation(s) 1 : Ifremer, france
2 : CNRS, france
3 : Univ perpignan, France
Source Bmc Genomics (1471-2164) (Research Square), 2020-01 , Vol. 21 , N. 1 , P. 63 (14p.)
DOI 10.1186/s12864-020-6471-x
WOS© Times Cited 22
Keyword(s) Pacific oyster, Oyster disease, Resistance, OsHV-1, Antiviral molecular pathways, Invertebrate immunity

Background: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. Conclusions: We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function.

Full Text
File Pages Size Access
Publisher's official version 39 1 MB Open access
Additional file 1: OsHV-1 and total vibrio colonization in oysters during the two mesocosm experiments 1 230 KB Open access
Additional file 2: List of DEGs shared by at least two resistant families. 941 KB Open access
Additional file 3: List of DEGs specifics to the RF21 oyster family. 412 KB Open access
Additional file 4: List of DEGs specifics to the RF23 oyster family 279 KB Open access
Additional file 5: List of DEGs specifics to the RF48 oyster family. 534 KB Open access
Additional file 6: Blast2GO annotation of the 28,027 genes identified in the genome of C. gigas. 2 MB Open access
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de Lorgeril Julien, Petton Bruno, Lucasson Aude, Perez Valérie, Stenger Pierre-Louis, Degremont Lionel, Montagnani Caroline, Escoubas Jean Michel, Haffner Philippe, Allienne Jean-François, Leroy Marc, Lagarde Franck, Vidal Dupiol Jeremie, Gueguen Yannick, Mitta Guillaume (2020). Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome. Bmc Genomics, 21(1), 63 (14p.). Publisher's official version : https://doi.org/10.1186/s12864-020-6471-x , Open Access version : https://archimer.ifremer.fr/doc/00602/71369/