Ostreopsis cf. ovata (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil

Type Article
Date 2020-02
Language English
Author(s) Nascimento Silvia M1, Neves Raquel Af1, De'Carli Gabriela Al1, Borsato Geovanna T1, Da Silva Rodrigo Af1, Melo Guilherme A2, De Morais Agatha M1, Cockell Thais C1, Fraga Santiago3, Menezes-Salgueiro Adriana D4, Mafra Luis L Jr5, Hess PhilippORCID6, Salgueiro Fabiano2
Affiliation(s) 1 : Laboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, Brazil
2 : Laboratório de Biodiversidade e Evolução Molecular, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 512, Rio de Janeiro, RJ 22290-240, Brazil
3 : Centro Oceanográfico de Vigo, Instituto Español de Oceanografía (IEO), Subida a Radio Faro 50, 36390 Vigo, Spain
4 : Laboratório de Biotecnologia Vegetal, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Rua Senador Furtado, 121, 112. Maracanã, Rio de Janeiro, RJ 20270-021, Brazil
5 : Centro de Estudos do Mar, Universidade Federal do Paraná, Cx. Postal 61, Pontal do Paraná, PR 83255-976, Brazil
6 : IFREMER, Laboratoire Phycotoxines, Rue de l’Ile d’Yeu, 44311 Nantes, France
Source Toxins (2072-6651) (MDPI), 2020-02 , Vol. 12 , N. 2 , P. 23p.
WOS© Times Cited 2
Note This article belongs to the Special Issue Potentially Toxic Benthic Microorganisms in Freshwater and Marine Ecosystems
Keyword(s) benthic dinoflagellates, harmful algal blooms, ovatoxins, taxonomy, morphometry
Abstract

Recurrent blooms of Ostreopsis cf. ovata have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1–D3 and D8–D10 regions of the LSU rDNA and ITS loci, and the morphology of O. cf. ovata isolates and field populations from locations along the Brazilian tropical and subtropical coastal regions and three oceanic islands are presented. Additional ITS sequences from three single cells from the tropical coast are provided. Toxin profiles and quantities of PLTX and their analogues; OVTXs; contained in cells from two clonal cultures and two field blooms from Rio de Janeiro were investigated. Morphology was examined using both light and epifluorescence microscopy. Morphometric analysis of different strains and field populations from diverse locations were compared. Molecular analysis showed that six of the seven sequences grouped at the large “Atlantic/Mediterranean/Pacific” sub-clade, while one sequence branched in a sister clade with sequences from Madeira Island and Greece. The toxin profile of strains and bloom field samples from Rio de Janeiro were dominated by OVTX-a and -b, with total cell quotas (31.3 and 39.3 pg cell−1) in the range of that previously reported for strains of O. cf. ovata.

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Nascimento Silvia M, Neves Raquel Af, De'Carli Gabriela Al, Borsato Geovanna T, Da Silva Rodrigo Af, Melo Guilherme A, De Morais Agatha M, Cockell Thais C, Fraga Santiago, Menezes-Salgueiro Adriana D, Mafra Luis L Jr, Hess Philipp, Salgueiro Fabiano (2020). Ostreopsis cf. ovata (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil. Toxins, 12(2), 23p. Open Access version : https://archimer.ifremer.fr/doc/00604/71594/