Zebrafish Danio rerio shows behavioural cross‐context consistency at larval and juvenile stages but no consistency between stages

Type Article
Date 2020-06
Language English
Author(s) Alfonso Sebastien1, 2, Peyrafort Manon1, Cousin XavierORCID2, 3, Bégout Marie-LaureORCID1, 2
Affiliation(s) 1 : Ifremer, Laboratoire Ressources HalieutiquesPlace Gaby Coll F‐17137 L'Houmeau, France
2 : MARBEC, Univ. Montpellier CNRS, Ifremer, IRD Chemin de Maguelone F‐34250 Chemin de Maguelone Palavas‐les‐flots, France
3 : Université Paris‐Saclay, INRAE, AgroParisTech, GABI Domaine de Vilvert Bâtiment 231, F‐78350 Jouy‐en‐Josas, France
Source Journal Of Fish Biology (0022-1112) (Wiley), 2020-06 , Vol. 96 , N. 6 , P. 1411-1421
DOI 10.1111/jfb.14310
WOS© Times Cited 1
Keyword(s) coping style, fish, personality, vital staining
Abstract

Coping style is defined as a set of individual physiological and behavioural characteristics consistent across time and context. In the zebrafish Danio rerio, as well as in many other animals, several covariations have been established between behavioural, physiological, and molecular responses. However, not many studies have addressed the consistency of behavioural responses over time starting at the larval stage. Therefore, we aimed to improve our understanding of behavioural consistency across context and over time in zebrafish from the larval to juvenile stages. We conducted two distinct experiments: a larval stage experiment (from 8 to 21 days post fertilization, dpf) and a juvenile stage experiment (from 21 to 60 dpf). On one hand, the larval experiment allows to focus on the transition between 8 and 21 dpf, marked by significant morphological changes related to the end of larval stage and initiation of metamorphosis. On the other hand, the juvenile experiment allows to properly cover the period extending from the end of larval stage to the juvenile stage (60 dpf) including metamorphosis which is itself completed around 45 dpf. Within each experiment, boldness was determined using a group risk‐taking test to identify bold and shy individuals. A novel environment test was then performed at the same age to evaluate consistency across context. Groups of fish (either bold or shy) were bathed in an alizarin red S solution for later identification of their initially determined coping style to evaluate behavioural consistency over time. Fish were then reared under common garden conditions and challenged again with the same behavioural tests at a later age (21 and 60 dpf in the larval and juvenile experiments, respectively). We observed behavioural consistency across context, with bold fish being more active and expressing higher thigmotaxis regardless of age. There was, however, little behavioural consistency over age, suggesting behavioural plasticity during development. Moreover, the use of alizarin red S to conduct this experiment provides new perspectives for the further study of the longitudinal evolution of various traits, including behaviour, over life stages in fish.

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Alfonso Sebastien, Peyrafort Manon, Cousin Xavier, Bégout Marie-Laure (2020). Zebrafish Danio rerio shows behavioural cross‐context consistency at larval and juvenile stages but no consistency between stages. Journal Of Fish Biology, 96(6), 1411-1421. Publisher's official version : https://doi.org/10.1111/jfb.14310 , Open Access version : https://archimer.ifremer.fr/doc/00614/72598/