Multiple independent chromosomal fusions accompanied the radiation of the Antarctic teleost genus Trematomus (Notothenioidei:Nototheniidae)

Type Article
Date 2020-03
Language English
Author(s) Auvinet Juliette1, 2, 3, Graça Paula1, 2, Dettai Agnès2, Amores Angel4, Postlethwait John H.4, Detrich H. William3, Ozouf-Costaz Catherine1, Coriton Olivier5, Higuet Dominique1, 2
Affiliation(s) 1 : Sorbonne Université, CNRS, Université des Antilles, Evolution Paris Seine - Institut de Biologie Paris Seine (EPS - IBPS), 75005, Paris, France
2 : Institut de Systématique, Evolution, Biodiversité (ISYEB) – Muséum National d’Histoire Naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, 43, rue Cuvier, 75231, Paris Cedex 05, France
3 : Department of Marine and Environmental Sciences, Marine Science Center, Northeastern University, Nahant, MA, 01908, USA
4 : Institute of Neuroscience, Department of Biology, University of Oregon, Eugene, OR, 97403, USA
5 : Institut National de Recherche pour l’agriculture, l’alimentation et l’environnement, INRAE, UMR1349 IGEPP, Molecular cytogenetics Platform, BP35327, F-35653, Le Rheu Cedex, France
Source Bmc Evolutionary Biology (1471-2148) (Springer Science and Business Media LLC), 2020-03 , Vol. 20 , N. 1 , P. 39 (14p.)
DOI 10.1186/s12862-020-1600-3
WOS© Times Cited 1
Keyword(s) BAC-FISH, Chromosomal painting, Chromosomal rearrangements, Chromosomal structural units, Chromosomal synteny, Nototheniidae, Speciation
Abstract

Background

Chromosomal rearrangements are thought to be an important driving force underlying lineage diversification, but their link to speciation continues to be debated. Antarctic teleost fish of the family Nototheniidae (Notothenioidei) diversified in a changing environmental context, which led to ecological, morphological, and genetic differentiation among populations. In addition, extensive chromosomal repatterning accompanied species divergence in several clades. The most striking karyotypic changes involved the recent species radiation (about 10 My) of the genus Trematomus, with chromosomal pair numbers ranging between 29 and 12. These dramatic reductions in chromosome number resulted mostly from large-scale chromosome fusions. Multiple centric and/or tandem fusions have been hypothesized in at least seven of the twelve recognized Trematomus species. To reconstruct their evolutionary history, we employed comparative cytogenomics (BAC-FISH and chromosome painting) to reveal patterns of interspecific chromosomal orthologies across several notothenioid clades.

Results

We defined orthologous chromosomal segments of reference, termed Structural Units (SUs). SUs were identified in a total of 18 notothenioid species. We demonstrated for the first time that SUs were strongly conserved across every specimen examined, with chromosomal syntenies highlighting a paucity of intrachromosomal macro-rearrangements. Multiple independent fusions of these SUs were inferred in the Trematomus species, in contrast to the shared SU fusions in species of the sister lineage Notothenia.

Conclusions

The SU segments were defined units of chromosomal rearrangement in the entire family Nototheiidae, which diverged from the other notothenioid families 20 My ago. Some of the identified chromosomal syntenies within the SUs were even conserved in their closest relatives, the family Eleginopsidae. Comparing the timing of acquisition of the fusions in the closely related genera Notothenia and Trematomus of the nototheniid species family, we conclude that they exhibit distinct chromosomal evolutionary histories, which may be relevant to different speciation scenarios.

Full Text
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Publisher's official version 14 5 MB Open access
Additional file 1. Complete list of the 40 BACs studied. The BACs are grouped according to the signal category: “specific”, “repetitive” or “weak”. SU locations, chromosomal positions and species ... 2 102 KB Open access
Additional file 2. Species sampling for chromosomal preparations and for tissue (muscle) for DNA extraction. This file contains a table describing all the specimens’ material used in this study. 2 103 KB Open access
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How to cite 

Auvinet Juliette, Graça Paula, Dettai Agnès, Amores Angel, Postlethwait John H., Detrich H. William, Ozouf-Costaz Catherine, Coriton Olivier, Higuet Dominique (2020). Multiple independent chromosomal fusions accompanied the radiation of the Antarctic teleost genus Trematomus (Notothenioidei:Nototheniidae). Bmc Evolutionary Biology, 20(1), 39 (14p.). Publisher's official version : https://doi.org/10.1186/s12862-020-1600-3 , Open Access version : https://archimer.ifremer.fr/doc/00615/72731/