Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata

Type Article
Date 2019-03
Language English
Author(s) Pinaud Silvain1, 4, Portet Anais1, Allienne Jean-Francois1, Belmudes LucidORCID2, Saint-Beat Cecile1, Arancibia Nathalie1, Galinier RichardORCID1, Du Pasquier Louis3, Duval David1, Gourbal Benjamin1
Affiliation(s) 1 : Univ Montpellier, Univ Perpignan, Interact Hates Pathogens Environm, UMR 5244,CNRS, Via Domitia, F-66860 Perpignan, France.
2 : CEA Grenoble Exploring Dynam Proteomes EDyP, F-38054 Grenoble 9, France.
3 : Univ Basel, Zool Inst, Dept Zool & Evolutionary Biol, Vesalgasse 1, Basel, Switzerland.
4 : Wellcome Trust Sanger Inst, Hinxton CB10 1SA, England.
Source Developmental And Comparative Immunology (0145-305X) (Elsevier Sci Ltd), 2019-03 , Vol. 92 , P. 238-252
DOI 10.1016/j.dci.2018.12.001
WOS© Times Cited 8
Keyword(s) Immunological memory, Specificity, Schistosoma, Biomphalaria, RNAseq, Proteomic, Vaccination
Abstract Invertebrate immune response may be primed by a current infection in a sustained manner, leading to the failure of a secondary infection with the same pathogen. The present study focuses on the Schistosomiasis vector snail Biomphalaria glabrata, in which a specific genotype-dependent immunological memory was demonstrated as a shift from a cellular to a humoral immune response. Herein, we investigate the complex molecular bases associated with this genotype-dependant immunological memory response. We demonstrate that Biomphalaria regulates a polymorphic set of immune recognition molecules and immune effector repertoires to respond to different strains of Schistosoma parasites. These results suggest a combinatorial usage of pathogen recognition receptors (PRRs) that distinguish different strains of parasites during the acquisition of immunological memory. Immunizations also show that snails become resistant after exposure to parasite extracts. Hemolymph transfer and a label-free proteomic analysis proved that circulating hemolymph compounds can be produced and released to more efficiently kill the newly encountered parasite of the same genetic lineage.
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Pinaud Silvain, Portet Anais, Allienne Jean-Francois, Belmudes Lucid, Saint-Beat Cecile, Arancibia Nathalie, Galinier Richard, Du Pasquier Louis, Duval David, Gourbal Benjamin (2019). Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata. Developmental And Comparative Immunology, 92, 238-252. Publisher's official version : https://doi.org/10.1016/j.dci.2018.12.001 , Open Access version : https://archimer.ifremer.fr/doc/00615/72738/