Molecular characterisation of immunological memory following homologous or heterologous challenges in the schistosomiasis vector snail, Biomphalaria glabrata
|Author(s)||Pinaud Silvain1, 4, Portet Anais1, Allienne Jean-Francois1, Belmudes Lucid2, Saint-Beat Cecile1, Arancibia Nathalie1, Galinier Richard1, Du Pasquier Louis3, Duval David1, Gourbal Benjamin1|
|Affiliation(s)||1 : Univ Montpellier, Univ Perpignan, Interact Hates Pathogens Environm, UMR 5244,CNRS, Via Domitia, F-66860 Perpignan, France.
2 : CEA Grenoble Exploring Dynam Proteomes EDyP, F-38054 Grenoble 9, France.
3 : Univ Basel, Zool Inst, Dept Zool & Evolutionary Biol, Vesalgasse 1, Basel, Switzerland.
4 : Wellcome Trust Sanger Inst, Hinxton CB10 1SA, England.
|Source||Developmental And Comparative Immunology (0145-305X) (Elsevier Sci Ltd), 2019-03 , Vol. 92 , P. 238-252|
|WOS© Times Cited||11|
|Keyword(s)||Immunological memory, Specificity, Schistosoma, Biomphalaria, RNAseq, Proteomic, Vaccination|
|Abstract||Invertebrate immune response may be primed by a current infection in a sustained manner, leading to the failure of a secondary infection with the same pathogen. The present study focuses on the Schistosomiasis vector snail Biomphalaria glabrata, in which a specific genotype-dependent immunological memory was demonstrated as a shift from a cellular to a humoral immune response. Herein, we investigate the complex molecular bases associated with this genotype-dependant immunological memory response. We demonstrate that Biomphalaria regulates a polymorphic set of immune recognition molecules and immune effector repertoires to respond to different strains of Schistosoma parasites. These results suggest a combinatorial usage of pathogen recognition receptors (PRRs) that distinguish different strains of parasites during the acquisition of immunological memory. Immunizations also show that snails become resistant after exposure to parasite extracts. Hemolymph transfer and a label-free proteomic analysis proved that circulating hemolymph compounds can be produced and released to more efficiently kill the newly encountered parasite of the same genetic lineage.|