High prevalence of Schistosoma haematobium x Schistosoma bovis hybrids in schoolchildren in Cote d'Ivoire

Type Article
Date 2020-03
Language English
Author(s) Angora Etienne K.1, 2, 3, Allienne Jean-Francois4, Rey Olivier4, Menan Herve3, Toure Andre O.5, Coulibaly Jean T.1, 2, 6, 7, Raso Giovanna1, 2, Yavo William3, N'Goran Eliezer K.6, 7, Utzinger Jurg1, 2, Balmer Oliver1, 2, Boissier Jerome4
Affiliation(s) 1 : Swiss Trop & Publ Hlth Inst, Basel, Switzerland.
2 : Univ Basel, Basel, Switzerland.
3 : Univ Felix Houphouet Boigny, Unite Format & Rech Sci Pharmaceut & Biol, Abidjan, Cote Ivoire.
4 : Univ Perpignan Via Domitia, IFREMER, Univ Montpellier, IHPE,CNRS, Perpignan, France.
5 : Inst Pasteur Cote Ivoire, Abidjan, Cote Ivoire.
6 : Univ Felix Houphouet Boigny, Unite Format & Rech Biosci, Abidjan, Cote Ivoire.
7 : Ctr Suisse Rech Sci Cote Ivoire, Abidjan, Cote Ivoire.
Source Parasitology (0031-1820) (Cambridge Univ Press), 2020-03 , Vol. 147 , N. 3 , P. 287-294
DOI 10.1017/S0031182019001549
WOS© Times Cited 9
Keyword(s) Cote d'Ivoire, hybrids, molecular analysis, Schistosoma bovis, Schistosoma haematobium
Abstract

Schistosomiasis is a neglected tropical disease, though it is highly prevalent in many parts of sub-Saharan Africa. While Schistosoma haematobium-bovis hybrids have been reported in West Africa, no data about Schistosoma hybrids in humans are available from Cote d'Ivoire. This study aimed to identify and quantify S. haematobium-bovis hybrids among schoolchildren in four localities of Cote d'Ivoire. Urine samples were collected and examined by filtration to detect Schistosoma eggs. Eggs were hatched and 503 miracidia were individually collected and stored on Whatman(R) FTA cards for molecular analysis. Individual miracidia were molecularly characterized by analysis of mitochondrial cox1 and nuclear internal transcribed spacer 2 (ITS 2) DNA regions. A mitochondrial cox1-based diagnostic polymerase chain reaction was performed on 459 miracidia, with 239 (52.1%) exhibiting the typical band for S. haematobium and 220 (47.9%) the S. bovis band. The cox1 and ITS 2 amplicons were Sanger sequenced from 40 randomly selected miracidia to confirm species and hybrids status. Among the 33 cox1 sequences analysed, we identified 15 S. haematobium sequences (45.5%) belonging to seven haplotypes and 18 S. bovis sequences (54.5%) belonging to 12 haplotypes. Of 40 ITS 2 sequences analysed, 31 (77.5%) were assigned to pure S. haematobium, four (10.0%) to pure S. bovis and five (12.5%) to S. haematobium-bovis hybrids. Our findings suggest that S. haematobium-bovis hybrids are common in Cote d'Ivoire. Hence, intense prospection of domestic and wild animals is warranted to determine whether zoonotic transmission occurs.

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Angora Etienne K., Allienne Jean-Francois, Rey Olivier, Menan Herve, Toure Andre O., Coulibaly Jean T., Raso Giovanna, Yavo William, N'Goran Eliezer K., Utzinger Jurg, Balmer Oliver, Boissier Jerome (2020). High prevalence of Schistosoma haematobium x Schistosoma bovis hybrids in schoolchildren in Cote d'Ivoire. Parasitology, 147(3), 287-294. Publisher's official version : https://doi.org/10.1017/S0031182019001549 , Open Access version : https://archimer.ifremer.fr/doc/00624/73604/