Efficient, fast and inexpensive bioassay to monitor benthic microalgae toxicity: Application to Ostreopsis species

Type Article
Date 2020-06
Language English
Author(s) Pavaux Anne-Sophie1, Ternon Eva2, Dufour Louison1, Marro Sophie1, Gémin Marin-Pierre3, Thomas Olivier P.4, Lemée Rodolphe1
Affiliation(s) 1 : Sorbonne Université, CNRS, Laboratoire d’Océanographie de Villefranche, LOV, F-06230, Villefranche-sur-Mer, France
2 : Université Côte d’Azur, CNRS, OCA, IRD, Géoazur, 250 rue Albert Einstein, 06560, Valbonne, France
3 : IFREMER - Laboratoire Phycotoxines (PHYC), Rue de l'Île d'Yeu, BP 21105, F- 44311, Nantes, France
4 : Marine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway, University Road, H91 TK33, Galway, Ireland
Source Aquatic Toxicology (0166-445X) (Elsevier BV), 2020-06 , Vol. 223 , P. 105485 (12p.)
DOI 10.1016/j.aquatox.2020.105485
WOS© Times Cited 11
Keyword(s) Ostreopsis spp., Anemia franciscana, Bioassay, Ecotoxicity, HPLC-HRMS

Even though HPLC-MS is commonly used to quantify the toxin content of Ostreopsis spp. cells, there is a need to develop easy-to-use toxicological tests to set thresholds during Ostreopsis spp. blooms. The crustacean Artemia has been widely used to evaluate the presence and toxicity of chemicals and biological contaminants and we anticipated that it could also be useful to test Ostreopsis spp. toxicity. Its relevance was first assessed by investigating the variability of the toxic effects among Ostreopsis spp. strains and throughout the dinoflagellate life cycle in combination with chemical analyses of the toxinic content by UHPLC-HRMS. After testing the toxicity of fractions prepared from Ostreopsis spp. cells, the known ova- and paly-toxins were not the only toxic metabolites to Artemia franciscana, indicating that other toxic compounds synthesized by Ostreopsis spp. still remain to be identified. To extend the bioassay to in situ monitoring, the toxicity of the benthic microalgal consortium was tested during a natural bloom of Ostreopsis cf. ovata in the NW Mediterranean Sea. The results highlight the accuracy and sensitivity of the ecotoxicological assay with Artemia franciscana to assess the toxicity of Ostreopsis spp. blooms.

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